Optimal Survival of Helicobacter Pylori Under Various Transport Conditions

Overview

Journal J Clin Microbiol

Specialty Microbiology

Date 1992 Jun 1

PMID 1624562

Citations 20

Authors

V Soltesz

B Zeeberg

T Wadstrom

Affiliations

Soon will be listed here.

Abstract

The ability of clinical isolates and type strains of Helicobacter pylori to survive in Stuart transport medium, isotonic saline solution, and urea-containing isotonic saline was evaluated. The influences of temperature (4, 10, 15, 20, and 30 degrees C) and holding time (6 to 48 h) and the effect of exposure to air on survival were also studied. The recovery rate of H. pylori was highest from Stuart transport medium in comparison with the recoveries from the other transport media tested. We found that at holding temperatures above 15 degrees C the organisms became noncultivable within 6 h or less, while they survived for 2 days or longer at 10 degrees C. The presence of urea at a concentration of 2% (wt/vol) in isotonic saline resulted in the loss of viability of the organisms tested.

Citing Articles

in Dyspepsia: Phenotypic and Genotypic Methods of Diagnosis.

Shetty V, Ballal M, Balaraju G, Shetty S, Pai G, Lingadakai R J Glob Infect Dis. 2018; 9(4):131-134.

PMID: 29302146 PMC: 5750435. DOI: 10.4103/jgid.jgid_52_17.

Centralized isolation of Helicobacter pylori from multiple centers and transport condition influences.

Gong Y, Li Y, Yang N, Li H, Guo F, Lin L World J Gastroenterol. 2015; 21(3):944-52.

PMID: 25624729 PMC: 4299348. DOI: 10.3748/wjg.v21.i3.944.

New transport medium for cultural recovery of Helicobacter pylori.

Cellini L, Di Campli E, Di Bartolomeo S, Bessa L, Baffoni M, Di Giulio M J Clin Microbiol. 2014; 52(12):4325-9.

PMID: 25320229 PMC: 4313306. DOI: 10.1128/JCM.02850-14.

Maintenance of Helicobacter pylori cultures in agar stabs.

Xu J, Czinn S, Blanchard T Helicobacter. 2010; 15(5):477-80.

PMID: 21083755 PMC: 3107710. DOI: 10.1111/j.1523-5378.2010.00769.x.

Liquid culture medium for the rapid cultivation of Helicobacter pylori from biopsy specimens.

Sainsus N, Cattori V, Lepadatu C, Hofmann-Lehmann R Eur J Clin Microbiol Infect Dis. 2008; 27(12):1209-17.

PMID: 18604574 DOI: 10.1007/s10096-008-0567-6.

References

Stuart R, TOSHACH S, PATSULA T . The problem of transport of specimens for culture of Gonococci. Can J Public Health. 1954; 45(2):73-83. View

Goodwin C, Armstrong J . Microbiological aspects of Helicobacter pylori (Campylobacter pylori). Eur J Clin Microbiol Infect Dis. 1990; 9(1):1-13. DOI: 10.1007/BF01969526. View

Ansorg R, von Recklinghausen G, Pomarius R, Schmid E . Evaluation of techniques for isolation, subcultivation, and preservation of Helicobacter pylori. J Clin Microbiol. 1991; 29(1):51-3. PMC: 269701. DOI: 10.1128/jcm.29.1.51-53.1991. View

Bode G, Malfertheiner P, DITSCHUNEIT H . Pathogenetic implications of ultrastructural findings in Campylobacter pylori related gastroduodenal disease. Scand J Gastroenterol Suppl. 1988; 142:25-39. View

Hartmann D, von Graevenitz A . A note on name, viability and urease tests of Campylobacter pylori. Eur J Clin Microbiol. 1987; 6(1):82-3. DOI: 10.1007/BF02097207. View

Hazell S, Markesich D, Evans D, Evans D, Graham D . Influence of media supplements on growth and survival of Campylobacter pylori. Eur J Clin Microbiol Infect Dis. 1989; 8(7):597-602. DOI: 10.1007/BF01968136. View

Owen R, On S, Costas M . The effect of cooling rate, freeze-drying suspending fluid and culture age on the preservation of Campylobacter pylori. J Appl Bacteriol. 1989; 66(4):331-7. DOI: 10.1111/j.1365-2672.1989.tb02486.x. View

McNulty C, Dent J, Curry A, Uff J, Ford G, Gear M . New spiral bacterium in gastric mucosa. J Clin Pathol. 1989; 42(6):585-91. PMC: 1141985. DOI: 10.1136/jcp.42.6.585. View

Owen R, On S, Costas M . Potential transport medium for Campylobacter pylori. J Clin Pathol. 1988; 41(12):1337-8. PMC: 1141772. DOI: 10.1136/jcp.41.12.1337-b. View

10.

Archer J, Romero S, Ritchie A, Hamacher M, Steiner B, BRYNER J . Characterization of an unclassified microaerophilic bacterium associated with gastroenteritis. J Clin Microbiol. 1988; 26(1):101-5. PMC: 266203. DOI: 10.1128/jcm.26.1.101-105.1988. View

11.

Faoagali J, Troughton D, Aitken J, Gwynne J . Campylobacter-like organisms in gastric biopsies: a Christchurch study. N Z Med J. 1986; 99(795):50-2. View

12.

Morgan D, Freedman R, Depew C, Kraft W . Growth of Campylobacter pylori in liquid media. J Clin Microbiol. 1987; 25(11):2123-5. PMC: 269424. DOI: 10.1128/jcm.25.11.2123-2125.1987. View

13.

Krajden S, Bohnen J, Anderson J, Kempston J, Fuksa M, Matlow A . Comparison of selective and nonselective media for recovery of Campylobacter pylori from antral biopsies. J Clin Microbiol. 1987; 25(6):1117-8. PMC: 269150. DOI: 10.1128/jcm.25.6.1117-1118.1987. View

14.

Romaniuk P, Zoltowska B, Trust T, Lane D, Olsen G, Pace N . Campylobacter pylori, the spiral bacterium associated with human gastritis, is not a true Campylobacter sp. J Bacteriol. 1987; 169(5):2137-41. PMC: 212113. DOI: 10.1128/jb.169.5.2137-2141.1987. View

15.

Buck G, Smith J . Medium supplementation for growth of Campylobacter pyloridis. J Clin Microbiol. 1987; 25(4):597-9. PMC: 266041. DOI: 10.1128/jcm.25.4.597-599.1987. View

16.

Goodwin C, Blincow E, Warren J, WATERS T, Sanderson C, Easton L . Evaluation of cultural techniques for isolating Campylobacter pyloridis from endoscopic biopsies of gastric mucosa. J Clin Pathol. 1985; 38(10):1127-31. PMC: 499453. DOI: 10.1136/jcp.38.10.1127. View

17.

Kjoller M, Fischer A, Justesen T . Transport conditions and number of biopsies necessary for culture of Helicobacter pylori. Eur J Clin Microbiol Infect Dis. 1991; 10(3):166-7. DOI: 10.1007/BF01964450. View