PGP4, an ATP Binding Cassette P-glycoprotein, Catalyzes Auxin Transport in Arabidopsis Thaliana Roots

Overview

Journal Plant Cell

Publisher Oxford University Press

Specialties Biology
Cell Biology

Date 2005 Oct 26

PMID 16243904

Citations 143

Authors

Kazuyoshi Terasaka

Joshua J Blakeslee

Boosaree Titapiwatanakun

Wendy A Peer

Anindita Bandyopadhyay

Srinivas N Makam

Ok Ran Lee

Elizabeth L Richards

Angus S Murphy

Fumihiko Sato

Kazufumi Yazaki

Affiliations

Soon will be listed here.

Abstract

Members of the ABC (for ATP binding cassette) superfamily of integral membrane transporters function in cellular detoxification, cell-to-cell signaling, and channel regulation. More recently, members of the multidrug resistance P-glycoprotein (MDR/PGP) subfamily of ABC transporters have been shown to function in the transport of the phytohormone auxin in both monocots and dicots. Here, we report that the Arabidopsis thaliana MDR/PGP PGP4 functions in the basipetal redirection of auxin from the root tip. Reporter gene studies showed that PGP4 was strongly expressed in root cap and epidermal cells. PGP4 exhibits apolar plasma membrane localization in the root cap and polar localization in tissues above. Root gravitropic bending and elongation as well as lateral root formation were reduced in pgp4 mutants compared with the wild type. pgp4 exhibited reduced basipetal auxin transport in roots and a small decrease in shoot-to-root transport consistent with a partial loss of the redirective auxin sink in the root. Seedlings overexpressing PGP4 exhibited increased shoot-to-root auxin transport. Heterologous expression of PGP4 in mammalian cells resulted in 1-N-naphthylthalamic acid-reversible net uptake of [3H]indole-3-acetic acid. These results indicate that PGP4 functions primarily in the uptake of redirected or newly synthesized auxin in epidermal root cells.

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References

Pighin J, Zheng H, Balakshin L, Goodman I, Western T, Jetter R . Plant cuticular lipid export requires an ABC transporter. Science. 2004; 306(5696):702-4. DOI: 10.1126/science.1102331. View

Borner G, Sherrier D, Weimar T, Michaelson L, Hawkins N, MacAskill A . Analysis of detergent-resistant membranes in Arabidopsis. Evidence for plasma membrane lipid rafts. Plant Physiol. 2004; 137(1):104-16. PMC: 548842. DOI: 10.1104/pp.104.053041. View

Ieiri I, Suzuki H, Kimura M, Takane H, Nishizato Y, Irie S . Influence of common variants in the pharmacokinetic genes (OATP-C, UGT1A1, and MRP2) on serum bilirubin levels in healthy subjects. Hepatol Res. 2004; 30(2):91-95. DOI: 10.1016/j.hepres.2004.07.005. View

Klein M, Geisler M, Suh S, Kolukisaoglu H, Azevedo L, Plaza S . Disruption of AtMRP4, a guard cell plasma membrane ABCC-type ABC transporter, leads to deregulation of stomatal opening and increased drought susceptibility. Plant J. 2004; 39(2):219-36. DOI: 10.1111/j.1365-313X.2004.02125.x. View

De Michelis M, Rasi-Caldogno F, Pugliarello M, Olivari C . Fusicoccin Binding to Its Plasma Membrane Receptor and the Activation of the Plasma Membrane H+-ATPase (III. Is There a Direct Interaction between the Fusicoccin Receptor and the Plasma Membrane H+-ATPase?). Plant Physiol. 1996; 110(3):957-964. PMC: 157795. DOI: 10.1104/pp.110.3.957. View

Alonso J, Stepanova A, Leisse T, Kim C, Chen H, Shinn P . Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science. 2003; 301(5633):653-7. DOI: 10.1126/science.1086391. View

Ishikawa H, Evans M . Novel software for analysis of root gravitropism: comparative response patterns of Arabidopsis wild-type and axr1 seedlings. Plant Cell Environ. 1997; 20(7):919-28. DOI: 10.1046/j.1365-3040.1997.d01-129.x. View

Smith A, Nourizadeh S, Peer W, Xu J, Bandyopadhyay A, Murphy A . Arabidopsis AtGSTF2 is regulated by ethylene and auxin, and encodes a glutathione S-transferase that interacts with flavonoids. Plant J. 2003; 36(4):433-42. DOI: 10.1046/j.1365-313x.2003.01890.x. View

Danese I, Haine V, Delrue R, Tibor A, Lestrate P, Stevaux O . The Ton system, an ABC transporter, and a universally conserved GTPase are involved in iron utilization by Brucella melitensis 16M. Infect Immun. 2004; 72(10):5783-90. PMC: 517599. DOI: 10.1128/IAI.72.10.5783-5790.2004. View

10.

Muller A, Guan C, Galweiler L, Tanzler P, Huijser P, Marchant A . AtPIN2 defines a locus of Arabidopsis for root gravitropism control. EMBO J. 1998; 17(23):6903-11. PMC: 1171038. DOI: 10.1093/emboj/17.23.6903. View

11.

Ketchum C, Schmidt W, Rajendrakumar G, Michaelis S, Maloney P . The yeast a-factor transporter Ste6p, a member of the ABC superfamily, couples ATP hydrolysis to pheromone export. J Biol Chem. 2001; 276(31):29007-11. DOI: 10.1074/jbc.M100810200. View

12.

Bennett M, Marchant A, GREEN H, May S, WARD S, Millner P . Arabidopsis AUX1 gene: a permease-like regulator of root gravitropism. Science. 1996; 273(5277):948-50. DOI: 10.1126/science.273.5277.948. View

13.

Brown D, London E . Functions of lipid rafts in biological membranes. Annu Rev Cell Dev Biol. 1999; 14:111-36. DOI: 10.1146/annurev.cellbio.14.1.111. View

14.

Perez-Victoria J, Chiquero M, Conseil G, Dayan G, di Pietro A, Barron D . Correlation between the affinity of flavonoids binding to the cytosolic site of Leishmania tropica multidrug transporter and their efficiency to revert parasite resistance to daunomycin. Biochemistry. 1999; 38(6):1736-43. DOI: 10.1021/bi982455v. View

15.

Swarup R, Kargul J, Marchant A, Zadik D, Rahman A, Mills R . Structure-function analysis of the presumptive Arabidopsis auxin permease AUX1. Plant Cell. 2004; 16(11):3069-83. PMC: 527199. DOI: 10.1105/tpc.104.024737. View

16.

Hou G, Kramer V, Wang Y, Chen R, Perbal G, Gilroy S . The promotion of gravitropism in Arabidopsis roots upon actin disruption is coupled with the extended alkalinization of the columella cytoplasm and a persistent lateral auxin gradient. Plant J. 2004; 39(1):113-25. DOI: 10.1111/j.1365-313X.2004.02114.x. View

17.

Clough S, Bent A . Floral dip: a simplified method for Agrobacterium-mediated transformation of Arabidopsis thaliana. Plant J. 1999; 16(6):735-43. DOI: 10.1046/j.1365-313x.1998.00343.x. View

18.

Marchant A, Kargul J, May S, Muller P, Delbarre A, Perrot-Rechenmann C . AUX1 regulates root gravitropism in Arabidopsis by facilitating auxin uptake within root apical tissues. EMBO J. 1999; 18(8):2066-73. PMC: 1171291. DOI: 10.1093/emboj/18.8.2066. View

19.

Anderson M, Gregory R, Thompson S, Souza D, Paul S, Mulligan R . Demonstration that CFTR is a chloride channel by alteration of its anion selectivity. Science. 1991; 253(5016):202-5. DOI: 10.1126/science.1712984. View

20.

Blakeslee J, Peer W, Murphy A . Auxin transport. Curr Opin Plant Biol. 2005; 8(5):494-500. DOI: 10.1016/j.pbi.2005.07.014. View