In Vitro and in Vivo Two-photon Luminescence Imaging of Single Gold Nanorods

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 2005 Oct 22

PMID 16239346

Citations 154

Authors

Haifeng Wang

Terry B Huff

Daniel A Zweifel

Wei He

Philip S Low

Alexander Wei

Ji-Xin Cheng

Affiliations

Soon will be listed here.

Abstract

Gold nanorods excited at 830 nm on a far-field laser-scanning microscope produced strong two-photon luminescence (TPL) intensities, with a cos(4) dependence on the incident polarization. The TPL excitation spectrum can be superimposed onto the longitudinal plasmon band, indicating a plasmon-enhanced two-photon absorption cross section. The TPL signal from a single nanorod is 58 times that of the two-photon fluorescence signal from a single rhodamine molecule. The application of gold nanorods as TPL imaging agents is demonstrated by in vivo imaging of single nanorods flowing in mouse ear blood vessels.

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References

Zweifel D, Wei A . Sulfide-Arrested Growth of Gold Nanorods. Chem Mater. 2007; 17(16):4256-4261. PMC: 1847745. DOI: 10.1021/cm0506858. View

Albota M, Xu C, Webb W . Two-Photon Fluorescence Excitation Cross Sections of Biomolecular Probes from 690 to 960 nm. Appl Opt. 2008; 37(31):7352-6. DOI: 10.1364/ao.37.007352. View

Larson D, Zipfel W, Williams R, Clark S, Bruchez M, Wise F . Water-soluble quantum dots for multiphoton fluorescence imaging in vivo. Science. 2003; 300(5624):1434-6. DOI: 10.1126/science.1083780. View

Magde D, Elson E, Webb W . Fluorescence correlation spectroscopy. II. An experimental realization. Biopolymers. 1974; 13(1):29-61. DOI: 10.1002/bip.1974.360130103. View

Anceau C, Brasselet S, Zyss J, Gadenne P . Local second-harmonic generation enhancement on gold nanostructures probed by two-photon microscopy. Opt Lett. 2003; 28(9):713-5. DOI: 10.1364/ol.28.000713. View

Sonnichsen C, Alivisatos A . Gold nanorods as novel nonbleaching plasmon-based orientation sensors for polarized single-particle microscopy. Nano Lett. 2005; 5(2):301-4. DOI: 10.1021/nl048089k. View

Yelin D, Oron D, Thiberge S, Moses E, Silberberg Y . Multiphoton plasmon-resonance microscopy. Opt Express. 2009; 11(12):1385-91. DOI: 10.1364/oe.11.001385. View

Imura K, Nagahara T, Okamoto H . Plasmon mode imaging of single gold nanorods. J Am Chem Soc. 2004; 126(40):12730-1. DOI: 10.1021/ja047836c. View

Boyd , Yu , Shen . Photoinduced luminescence from the noble metals and its enhancement on roughened surfaces. Phys Rev B Condens Matter. 1986; 33(12):7923-7936. DOI: 10.1103/physrevb.33.7923. View

10.

El-Sayed M . Some interesting properties of metals confined in time and nanometer space of different shapes. Acc Chem Res. 2001; 34(4):257-64. DOI: 10.1021/ar960016n. View

11.

Sonnichsen C, Franzl T, Wilk T, von Plessen G, Feldmann J, Wilson O . Drastic reduction of plasmon damping in gold nanorods. Phys Rev Lett. 2002; 88(7):077402. DOI: 10.1103/PhysRevLett.88.077402. View

12.

Berland K, So P, Gratton E . Two-photon fluorescence correlation spectroscopy: method and application to the intracellular environment. Biophys J. 1995; 68(2):694-701. PMC: 1281733. DOI: 10.1016/S0006-3495(95)80230-4. View