Upregulation of T-bet Expression in Peripheral Blood Mononuclear Cells During Vogt-Koyanagi-Harada Disease

Overview

Journal Br J Ophthalmol

Specialty Ophthalmology

Date 2005 Oct 20

PMID 16234441

Citations 13

Authors

B Li

P Yang

H Zhou

X Huang

H Jin

L Chu

Y Gao

L Zhu

A Kijlstra

Affiliations

Soon will be listed here.

Abstract

Aim: To test the hypothesis that T-bet expression is altered in patients with Vogt-Koyanagi-Harada (VKH) disease.

Methods: Peripheral blood was withdrawn from 16 VKH patients before and after immunosuppressive treatment and from 16 healthy individuals. IFN-gamma, IL-2, and IL-4 in the serum and the supernatants of peripheral blood mononuclear cells (PBMC) cultured with or without phytohaemagglutinin (PHA) were measured by ELISA. T-bet mRNA and protein expression in PBMC cultured with or without PHA was detected by RT-PCR and western blot, respectively.

Results: The level of IFN-gamma, but not IL-2 and IL-4, was significantly higher in the supernatants of stimulated PBMC in patients than in controls. A significantly increased T-bet mRNA was found in VKH patients during an active uveitis episode, but not in quiescent patients, compared to controls. T-bet protein was detectable in VKH patients during an active uveitis episode, but not in quiescent patients nor in the healthy controls. Stimulation of PBMC with PHA resulted in a marked upregulation of T-bet mRNA and protein expression for both patients and controls with no significant difference between the two groups.

Conclusions: Upregulation of T-bet may be associated with the development of a Th1 mediated immune response in VKH disease.

Citing Articles

Identification of immune hub genes participating in the pathogenesis and progression of Vogt-Koyanagi-Harada disease.

Wang Y, Ju Y, Wang J, Sun N, Tang Z, Gao H Front Immunol. 2022; 13:936707.

PMID: 35958546 PMC: 9358976. DOI: 10.3389/fimmu.2022.936707.

Chromatin accessibility analysis reveals regulatory dynamics and therapeutic relevance of Vogt-Koyanagi-Harada disease.

Shi W, Ye J, Shi Z, Pan C, Zhang Q, Lin Y Commun Biol. 2022; 5(1):506.

PMID: 35618758 PMC: 9135711. DOI: 10.1038/s42003-022-03430-9.

Analyses of circRNA and mRNA Profiles in Vogt-Koyanagi-Harada Disease.

Shu J, Su G, Zhang J, Liu Z, Chang R, Wang Q Front Immunol. 2022; 12:738760.

PMID: 35003060 PMC: 8727692. DOI: 10.3389/fimmu.2021.738760.

miRNA Landscape in Pathogenesis and Treatment of Vogt-Koyanagi-Harada Disease.

Vega-Tapia F, Bustamante M, Valenzuela R, Urzua C, Cuitino L Front Cell Dev Biol. 2021; 9:658514.

PMID: 34041239 PMC: 8141569. DOI: 10.3389/fcell.2021.658514.

Case Series: Gene Expression Analysis in Canine Vogt-Koyanagi-Harada/Uveodermatologic Syndrome and Vitiligo Reveals Conserved Immunopathogenesis Pathways Between Dog and Human Autoimmune Pigmentary Disorders.

Egbeto I, Garelli C, Piedra-Mora C, Wong N, David C, Robinson N Front Immunol. 2021; 11:590558.

PMID: 33384688 PMC: 7770226. DOI: 10.3389/fimmu.2020.590558.

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