Distinct Enzyme Combinations in AKAP Signalling Complexes Permit Functional Diversity

Overview

Journal Nat Cell Biol

Specialty Cell Biology

Date 2005 Oct 18

PMID 16228013

Citations 104

Authors

Naoto Hoshi

Lorene K Langeberg

John D Scott

Affiliations

Soon will be listed here.

Abstract

Specificity in cell signalling can be influenced by the targeting of different enzyme combinations to substrates. The A-kinase anchoring protein AKAP79/150 is a multivalent scaffolding protein that coordinates the subcellular localization of second-messenger-regulated enzymes, such as protein kinase A, protein kinase C and protein phosphatase 2B. We developed a new strategy that combines RNA interference of the endogenous protein with a protocol that selects cells that have been rescued with AKAP79/150 forms that are unable to anchor selected enzymes. Using this approach, we show that AKAP79/150 coordinates different enzyme combinations to modulate the activity of two distinct neuronal ion channels: AMPA-type glutamate receptors and M-type potassium channels. Utilization of distinct enzyme combinations in this manner provides a means to expand the repertoire of cellular events that the same AKAP modulates.

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