An Aptamer That Neutralizes R5 Strains of Human Immunodeficiency Virus Type 1 Blocks Gp120-CCR5 Interaction

Overview

Journal J Virol

Publisher American Society for Microbiology

Date 2005 Oct 18

PMID 16227301

Citations 37

Authors

Antu K Dey

Makobetsa Khati

Min Tang

Richard Wyatt

Susan M Lea

William James

Affiliations

Soon will be listed here.

Abstract

We recently described the isolation and structural characterization of 2'-fluoropyrimidine-substituted RNA aptamers that bind to gp120 of R5 strains of human immunodeficiency virus type 1 and thereby potently neutralize the infectivity of phylogenetically diverse R5 strains. Here we investigate the physical basis of their antiviral action. We show that both N-linked oligosaccharides and the variable loops V1/V2 and V3 are not required for binding of one aptamer, B40, to gp120. Using surface plasmon resonance binding analyses, we show that the aptamer binds to the CCR5-binding site on gp120 in a relatively CD4-independent manner, providing a mechanistic explanation for its neutralizing potency.

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