Novel Fluorogenic Substrates for Imaging Beta-lactamase Gene Expression

Overview

Journal J Am Chem Soc

Publisher American Chemical Society

Specialty Chemistry

Date 2005 Oct 14

PMID 16220906

Citations 41

Authors

Wenzhong Gao

Bengang Xing

Roger Y Tsien

Jianghong Rao

Affiliations

Soon will be listed here.

Abstract

A new class of small nonfluorescent fluorogenic substrates becomes brightly fluorescent after beta-lactamase hydrolysis with up to 153-fold enhancement in the fluorescence intensity. Less than 500 fM of beta-lactamase in cell lysates can be readily detected, and beta-lactamase expression in living cells can be imaged with a red fluorescence derivative. These new fluorogenic substrates should find uses in clinical diagnostics and facilitate the applications of beta-lactamase as a biosensor.

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