The Role of Extracellular Matrix in Peripheral Nerve Regeneration: a Wound Chamber Study
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A wound chamber model was used for the study of the interaction between axon, Schwann cell and extracellular matrix during peripheral nerve regeneration. Impermeable silicone tubes, 8 mm long and 1.4 mm in internal diameter were sutured to transected rat sciatic nerve and the contents of the tubes were removed at intervals for chemical, histological, immunocytochemical and electron microscopic studies. There was an initial phase of fluid accumulation and the formation of a fibrin/fibronectin clot or cable which connected the cut ends of the nerve. The chamber fluid was shown to have a protein profile similar to that of rat serum. Schwann cells, endothelial cells and fibroblasts migrated first into the cable, apparently mediated by cell-fibrin interaction. Axons buried within the Schwann cell cytoplasm were led into the cable but an axon-fibrin interaction was not observed. After 1 week, the fibrin matrix underwent dissolution, with replacement by collagen. This marked the onset of myelination and the organization of nerve fibers into fascicles. The findings from the present study suggest that the interactions between axon and Schwann cell and between Schwann cell and a changing extracellular matrix are the essential driving force in nerve growth and differentiation during peripheral nerve regeneration.
near-infrared fluorescent fibrin highlights growth of nerve during regeneration across a nerve gap.
Luzhansky I, Anisman E, Patel D, Syed N, Wood M, Berezin M J Biomed Opt. 2022; 27(7):070502.
PMID: 36451699 PMC: 9297728. DOI: 10.1117/1.JBO.27.7.070502.
Incorporating Blood Flow in Nerve Injury and Regeneration Assessment.
Yeoh S, Warner W, Merchant S, Hsu E, Agoston D, Mahan M Front Surg. 2022; 9:862478.
PMID: 35529911 PMC: 9069240. DOI: 10.3389/fsurg.2022.862478.
Boso D, Maghin E, Carraro E, Giagante M, Pavan P, Piccoli M Materials (Basel). 2020; 13(11).
PMID: 32486040 PMC: 7321144. DOI: 10.3390/ma13112483.
Kimura H, Ouchi T, Shibata S, Amemiya T, Nagoshi N, Nakagawa T Sci Rep. 2018; 8(1):10071.
PMID: 29968745 PMC: 6030210. DOI: 10.1038/s41598-018-27952-7.
Cheng C, Solorio L, Alsberg E Biotechnol Adv. 2014; 32(2):462-84.
PMID: 24417915 PMC: 3959761. DOI: 10.1016/j.biotechadv.2013.12.012.