Chromosome-size Variation in Giardia Lamblia: the Role of RDNA Repeats

Overview

Journal Nucleic Acids Res

Publisher Oxford University Press

Specialty Biochemistry

Date 1992 Jun 25

PMID 1620602

Citations 24

Authors

R D Adam

Affiliations

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Abstract

Giardia lamblia trophozoites contain at least five sets of chromosomes that have been categorized by chromosome-specific probes. Pulsed field separations of G. lamblia chromosomes also demonstrated minor bands in some isolates which stained less intensely with ethidium than the major chromosomal bands. Two of the minor bands of the E11 clone of the ISR isolate, MBa and MBb, were similar to each other and to chromosomal band I by hybridization to total chromosomal DNA and by hybridization of specific probes. In order to determine the extent of this similarity, I have developed a panel of probes for many of the Pacl restriction fragments and have shown that most of the Pacl and Notl fragments found in MBa are also present in MBb. The differences are found in both telomeric regions. At one end, MBb contains a 300 kb region not found in MBa. At the other end of MBb is a 160 kb region containing the rDNA repeats which is bounded on one end by the telomeric repeat and on the other by sites for multiple enzymes that do not digest the rDNA repeats. The corresponding region of MBa is 23 kb in size. The size difference is consistent with the eightfold greater number of rDNA repeats in MBb than MBa and suggests that 30% of the size difference is accounted for by different numbers of copies of the rDNA repeat. MBa of another ISR clone (ISR G5) is 150 kb larger in size than MBa of ISR E11. The data suggest that MBa and MBb are homologous chromosomes of different sizes and that a portion of the size difference is accounted for by different copy numbers of the rDNA repeat.

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References

Keister D . Axenic culture of Giardia lamblia in TYI-S-33 medium supplemented with bile. Trans R Soc Trop Med Hyg. 1983; 77(4):487-8. DOI: 10.1016/0035-9203(83)90120-7. View

Wiesehahn G, Jarroll E, Lindmark D, Meyer E, Hallick L . Giardia lamblia: autoradiographic analysis of nuclear replication. Exp Parasitol. 1984; 58(1):94-100. DOI: 10.1016/0014-4894(84)90024-9. View

Le Blancq S, Korman S, Van der Ploeg L . Frequent rearrangements of rRNA-encoding chromosomes in Giardia lamblia. Nucleic Acids Res. 1991; 19(16):4405-12. PMC: 328627. DOI: 10.1093/nar/19.16.4405. View

Wellems T, Panton L . Genetic mapping of the chloroquine-resistance locus on Plasmodium falciparum chromosome 7. Proc Natl Acad Sci U S A. 1991; 88(8):3382-6. PMC: 51451. DOI: 10.1073/pnas.88.8.3382. View

Le Blancq S, Kase R, Van der Ploeg L . Analysis of a Giardia lamblia rRNA encoding telomere with [TAGGG]n as the telomere repeat. Nucleic Acids Res. 1991; 19(20):5790. PMC: 328996. DOI: 10.1093/nar/19.20.5790. View

Adam R, Nash T, Wellems T . Telomeric location of Giardia rDNA genes. Mol Cell Biol. 1991; 11(6):3326-30. PMC: 360185. DOI: 10.1128/mcb.11.6.3326-3330.1991. View

Fan J, Korman S, Cantor C, Smith C . Giardia lamblia: haploid genome size determined by pulsed field gel electrophoresis is less than 12 Mb. Nucleic Acids Res. 1991; 19(8):1905-8. PMC: 328122. DOI: 10.1093/nar/19.8.1905. View

Nash T, McCutchan T, Keister D, Dame J, Conrad J, Gillin F . Restriction-endonuclease analysis of DNA from 15 Giardia isolates obtained from humans and animals. J Infect Dis. 1985; 152(1):64-73. DOI: 10.1093/infdis/152.1.64. View

Boothroyd J, Wang A, Campbell D, Wang C . An unusually compact ribosomal DNA repeat in the protozoan Giardia lamblia. Nucleic Acids Res. 1987; 15(10):4065-84. PMC: 340832. DOI: 10.1093/nar/15.10.4065. View

10.

Adam R, Nash T, Wellems T . The Giardia lamblia trophozoite contains sets of closely related chromosomes. Nucleic Acids Res. 1988; 16(10):4555-67. PMC: 336648. DOI: 10.1093/nar/16.10.4555. View

11.

Edlind T, Chakraborty P . Unusual ribosomal RNA of the intestinal parasite Giardia lamblia. Nucleic Acids Res. 1987; 15(19):7889-901. PMC: 306315. DOI: 10.1093/nar/15.19.7889. View

12.

Pologe L, Ravetch J . Large deletions result from breakage and healing of P. falciparum chromosomes. Cell. 1988; 55(5):869-74. DOI: 10.1016/0092-8674(88)90142-0. View

13.

Sinnis P, Wellems T . Long-range restriction maps of Plasmodium falciparum chromosomes: crossingover and size variation among geographically distant isolates. Genomics. 1988; 3(4):287-95. DOI: 10.1016/0888-7543(88)90117-6. View

14.

Cappai R, van Schravendijk M, Anders R, Peterson M, Thomas L, Cowman A . Expression of the RESA gene in Plasmodium falciparum isolate FCR3 is prevented by a subtelomeric deletion. Mol Cell Biol. 1989; 9(8):3584-7. PMC: 362412. DOI: 10.1128/mcb.9.8.3584-3587.1989. View

15.

Giannini S, Schittini M, Keithly J, Warburton P, Cantor C, Van der Ploeg L . Karyotype analysis of Leishmania species and its use in classification and clinical diagnosis. Science. 1986; 232(4751):762-5. DOI: 10.1126/science.3961502. View

16.

Kabnick K, Peattie D . In situ analyses reveal that the two nuclei of Giardia lamblia are equivalent. J Cell Sci. 1990; 95 ( Pt 3):353-60. DOI: 10.1242/jcs.95.3.353. View

17.

Healey A, Mitchell R, Upcroft J, Boreham P, Upcroft P . Complete nucleotide sequence of the ribosomal RNA tandem repeat unit from Giardia intestinalis. Nucleic Acids Res. 1990; 18(13):4006. PMC: 331122. DOI: 10.1093/nar/18.13.4006. View

18.

Feinberg A, Vogelstein B . A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Anal Biochem. 1983; 132(1):6-13. DOI: 10.1016/0003-2697(83)90418-9. View

19.

Carle G, Olson M . Separation of chromosomal DNA molecules from yeast by orthogonal-field-alternation gel electrophoresis. Nucleic Acids Res. 1984; 12(14):5647-64. PMC: 320021. DOI: 10.1093/nar/12.14.5647. View

20.

Gottesdiener K, Garcia-Anoveros J, Lee M, Van der Ploeg L . Chromosome organization of the protozoan Trypanosoma brucei. Mol Cell Biol. 1990; 10(11):6079-83. PMC: 361410. DOI: 10.1128/mcb.10.11.6079-6083.1990. View