Identification of a Site Required for DNA Replication Fork Blocking Activity in the RRNA Gene Cluster in Saccharomyces Cerevisiae

Overview

Journal Mol Gen Genet

Specialties Genetics
Molecular Biology

Date 1992 Jun 1

PMID 1620093

Citations 50

Authors

T Kobayashi

M Hidaka

M Nishizawa

T Horiuchi

Affiliations

Soon will be listed here.

Abstract

The yeast genome has DNA replication fork blocking sites, that we have named sog sites, in the ribosomal RNA gene (rDNA) cluster. These are located at the 3' end of the 35S rRNA transcription unit and they block replication fork movement in a direction opposite to that of RNA polymerase I. We cloned this replication blocking site into a YEp-type plasmid and analyzed DNA replication intermediates, using two-dimensional (2D) agarose gel electrophoresis. The blocking activity remained even on a plasmid not involved in 35S rRNA transcription and inhibited fork movement in the same polar fashion as on the yeast chromosome. To define the site further, smaller fragments were subcloned into the YEp-type plasmid. A small 109 bp region exhibited sog activity and was located near the enhancer region for 35S rRNA transcription. It overlaps an essential element of the recombinational hot spot HOT1.

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