A Critical Evaluation of Neoglycoprotein Binding Sites in Vivo and in Sections of Mouse Tissues

Overview

Journal Histochemistry

Specialty Biochemistry

Date 1992 Jan 1

PMID 1618642

Citations 1

Authors

U Schumacher

Affiliations

Soon will be listed here.

Abstract

Endogenous lectins are reported to play a vital role in cell to cell communication. Their distribution in tissues has been widely studied by the use of labelled neoglycoproteins. In the present study, labelled neoglycoproteins were used on fixed and unfixed tissue sections and the results were compared with those observed after i.v. application of neoglycoproteins in mice. The study indicates that neoglycoprotein binding to tissue sections is not inhibited by application of the simple monosaccharides that were used to synthesize them. Furthermore the binding of neoglycoproteins following i.v. application into mice is rather limited. It is concluded that neoglycoproteins, which are synthesized using simple monosaccharides, do not provide a sensible tool to detect endogenous lectins in animal tissue sections. This is in sharp contrast to the results of most other studies reported in the literature.

Citing Articles

Visualization of carbohydrate-binding molecules expressed by myelomono- and erythropoietic cells derived from human bone marrow: an immunoenzymatic double-staining study.

Baldus S, Wickenhauser C, DITTMAR E, Stefanovic A, Thiele J Histochem J. 2000; 32(1):27-32.

PMID: 10805382 DOI: 10.1023/a:1003954111600.

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