Uptake and Intracellular Transport of Cationic Ferritin in the Bronchiolar and Alveolar Epithelia of the Rat

Overview

Journal Cell Tissue Res

Specialties Anatomy & Histology
Cell Biology

Date 1992 May 1

PMID 1617702

Citations 2

Authors

T Ito

H Kitamura

Y Inayama

A Nozawa

M KANISAWA

Affiliations

Soon will be listed here.

Abstract

Cationic ferritin was used as a marker to reveal the processes of endocytosis and intracellular transport in bronchiolar and alveolar epithelia. The marker was injected into the lung via the trachea, and ultrastructural observation of the distribution of ferritin particles in bronchiolar and alveolar epithelial cells was carried out at intervals of 5, 15, 30 and 60 min after the injection. The luminal surface of the airway and the alveolar epithelium showed diffuse labeling with cationic ferritin. In general, ferritin particles were observed in vesicles and vacuoles of the bronchiolar and alveolar epithelial cells within 5 min of injection; they appeared in multivesicular bodies within 15 min. Multivesicular bodies and secondary lysosomes containing ferritin particles, some of which showed a positive reaction for acid phosphatase, were seen in the basal cytoplasm within 30 min; ferritin particles appeared in the basal lamina below the Clara cells, ciliated cells and type 2 alveolar cells within 30 min. Ferritin particles were seen in ovoid granules of some Clara cells and in lamellar inclusion bodies of many type 2 alveolar cells. Brush cells and type 1 alveolar cells took up only a small quantity of ferritin particles.

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References

Ranga V, KLEINERMAN J . The effect of pilocarpine on vesicular uptake and transport of horseradish peroxidase by the guinea pig tracheal epithelium. Am Rev Respir Dis. 1982; 125(5):579-85. DOI: 10.1164/arrd.1982.125.5.579. View

Kalina M, Socher R . Internalization of pulmonary surfactant into lamellar bodies of cultured rat pulmonary type II cells. J Histochem Cytochem. 1990; 38(4):483-92. DOI: 10.1177/38.4.2156921. View

Schneeberger E, Karnovsky M . The influence of intravascular fluid volume on the permeability of newborn and adult mouse lungs to ultrastructural protein tracers. J Cell Biol. 2009; 49(2):319-34. PMC: 2108318. DOI: 10.1083/jcb.49.2.319. View

Bignon J, Chahinian P, Feldmann G, Sapin C . Ultrastructural immunoperoxidase demonstration of autologous albumin in the alveolar capillary membrane and in the alveolar lining material in normal rats. J Cell Biol. 1975; 64(2):503-9. PMC: 2109497. DOI: 10.1083/jcb.64.2.503. View

Silverstein S, Steinman R, COHN Z . Endocytosis. Annu Rev Biochem. 1977; 46:669-722. DOI: 10.1146/annurev.bi.46.070177.003321. View

Trier J, Allan C, Marcial M, Madara J . Structural features of the apical and tubulovesicular membranes of rodent small intestinal tuft cells. Anat Rec. 1987; 219(1):69-77. DOI: 10.1002/ar.1092190112. View

Williams M . Uptake of lectins by pulmonary alveolar type II cells: subsequent deposition into lamellar bodies. Proc Natl Acad Sci U S A. 1984; 81(20):6383-7. PMC: 391928. DOI: 10.1073/pnas.81.20.6383. View

Williams M . Endocytosis in alveolar type II cells: effect of charge and size of tracers. Proc Natl Acad Sci U S A. 1984; 81(19):6054-8. PMC: 391857. DOI: 10.1073/pnas.81.19.6054. View

LAUWERYNS J, Baert J . The role of the pulmonary lymphatics in the defenses of the distal lung: morphological and experimental studies of the transport mechanisms of intratracheally instillated particles. Ann N Y Acad Sci. 1974; 221:244-75. DOI: 10.1111/j.1749-6632.1974.tb28225.x. View

10.

Iseki S, Kondo H . An immunocytochemical study on the occurrence of liver fatty-acid-binding protein in the digestive organs of rats: specific localization in the D cells and brush cells. Acta Anat (Basel). 1990; 138(1):15-23. DOI: 10.1159/000146915. View

11.

Mostov K, Simister N . Transcytosis. Cell. 1985; 43(2 Pt 1):389-90. DOI: 10.1016/0092-8674(85)90166-7. View

12.

Gail D, Lenfant C . Cells of the lung: biology and clinical implications. Am Rev Respir Dis. 1983; 127(3):366-87. DOI: 10.1164/arrd.1983.127.3.366. View

13.

Rhodin J, DALHAMN T . Electron microscopy of the tracheal ciliated mucosa in rat. Z Zellforsch Mikrosk Anat. 1956; 44(4):345-412. DOI: 10.1007/BF00345847. View

14.

Petrik P, Collet A . Quantitative electron microscopic autoradiography of in vivo incorporation of 3H-choline, 3H-leucine, 3H-acetate and 3H-galactose in non-ciliated bronchiolar (Clara) cells of mice. Am J Anat. 1974; 139(4):519-33. DOI: 10.1002/aja.1001390405. View

15.

Richardson J, Bouchard T, FERGUSON C . Uptake and transport of exogenous proteins by respiratory epithelium. Lab Invest. 1976; 35(4):307-14. View

16.

Farquhar M . Multiple pathways of exocytosis, endocytosis, and membrane recycling: validation of a Golgi route. Fed Proc. 1983; 42(8):2407-13. View

17.

Matlin K, Reggio H, Helenius A, Simons K . Infectious entry pathway of influenza virus in a canine kidney cell line. J Cell Biol. 1981; 91(3 Pt 1):601-13. PMC: 2112819. DOI: 10.1083/jcb.91.3.601. View

18.

DeFouw D . Ultrastructural features of alveolar epithelial transport. Am Rev Respir Dis. 1983; 127(5 Pt 2):S9-13. View

19.

SPICER S, Schulte B, Thomopoulos G . Histochemical properties of the respiratory tract epithelium in different species. Am Rev Respir Dis. 1983; 128(2 Pt 2):S20-6. DOI: 10.1164/arrd.1983.128.2P2.S20. View

20.

Gil J . Number and distribution of plasmalemmal vesicles in the lung. Fed Proc. 1983; 42(8):2414-8. View