The Change of Proliferating Cell Nuclear Antigen and Apoptosis of the MM46 Mammary Cancer Cells of the Mouse After Single High-dose Irradiation

Overview

Journal Med Mol Morphol

Publisher Springer

Specialties Cell Biology
Molecular Biology
Pathology

Date 2005 Sep 20

PMID 16170466

Citations 1

Authors

Michiya Kobayashi

Ken Okamoto

Tsutomu Namikawa

Takehiro Okabayashi

Keijiro Araki

Affiliations

Soon will be listed here.

Abstract

The effects of intraoperative radiotherapy on tumor cells were elucidated by immunohistochemical examination of changes in the level of proliferating cell nuclear antigen. In addition, the terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling method was used to examine the level of apoptosis in mouse MM46 tumor cells after a single high dose of irradiation (30 Gy, 6 MeV). A significant decrease in the number of tumor cells compared to controls was observed on the 3rd, 7th, and 14th days following irradiation, but not on the 1st day. Consistent with these results, the proliferating cell nuclear antigen labeling index of irradiated cells decreased significantly on the 3rd, 7th, and 14th days following irradiation, but not on the 1st day. By comparison, the regrowth area on day 14 only showed no difference compared to the control group. The apoptotic index increased on the 7th and 14th day after irradiation, but at a lower rate than the observed decrease in the level of proliferating cell nuclear antigen. We speculate that the major mechanism of single high-dose radiation effect is inhibition of DNA synthesis.

Citing Articles

Scanning electron microscopic study of the three-dimensional structure of the collagen sheath surrounding cancer cells after single high-dose irradiation.

Kobayashi M, Furuya Y, Okabayashi T, Araki K Med Mol Morphol. 2006; 39(2):106-12.

PMID: 16821148 DOI: 10.1007/s00795-005-0308-1.

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