Direct Application of the INNO-LiPA Rif.TB Line-probe Assay for Rapid Identification of Mycobacterium Tuberculosis Complex Strains and Detection of Rifampin Resistance in 360 Smear-positive Respiratory Specimens from an Area of High Incidence Of...

Overview

Journal J Clin Microbiol

Specialty Microbiology

Date 2005 Sep 8

PMID 16145166

Citations 21

Authors

Miguel Viveiros

Clara Leandro

Liliana Rodrigues

Josefina Almeida

Rosario Bettencourt

Isabel Couto

Lurdes Carrilho

Jose Diogo

Ana Fonseca

Luis Lito

Joao Lopes

Teresa Pacheco

Mariana Pessanha

Judite Quirim

Luisa Sancho

Max Salfinger

Leonard Amaral

Affiliations

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Abstract

The INNO-LiPA Rif.TB assay for the identification of Mycobacterium tuberculosis complex strains and the detection of rifampin (RIF) resistance has been evaluated with 360 smear-positive respiratory specimens from an area of high incidence of multidrug-resistant tuberculosis (MDR-TB). The sensitivity when compared to conventional identification/culture methods was 82.2%, and the specificity was 66.7%; the sensitivity and specificity were 100.0% and 96.9%, respectively, for the detection of RIF resistance. This assay has the potential to provide rapid information that is essential for the effective management of MDR-TB.

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