NMR Analysis of Plant Nitrogen Metabolism

Overview

Journal Photosynth Res

Publisher Springer

Date 2005 Sep 7

PMID 16143850

Citations 13

Authors

F Mesnard

R G Ratcliffe

Affiliations

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Abstract

The analysis of primary and secondary nitrogen metabolism in plants by nuclear magnetic resonance (NMR) spectroscopy is comprehensively reviewed. NMR is a versatile analytical tool, and the combined use of (1)H, (2)H, (13)C, (14)N and (15)N NMR allows detailed investigation of the acquisition, assimilation and metabolism of nitrogen. The analysis of tissue extracts can be complemented by the in vivo NMR analysis of functioning tissues and cell suspensions, and by the application of solid state NMR techniques. Moreover stable isotope labelling with (2)H-, (13)C- and (15)N-labelled precursors provides direct insight into specific pathways, with the option of both time-course and steady state analysis increasing the potential value of the approach. The scope of the NMR method, and its contribution to studies of plant nitrogen metabolism, are illustrated with a wide range of examples. These include studies of the GS/GOGAT pathway of ammonium assimilation, investigations of the metabolism of glutamate, glycine and other amino acids, and applications to tropane alkaloid metabolism. The continuing development of the NMR technique, together with potential applications in the emerging fields of metabolomics and metabolic flux analysis, leads to the conclusion that NMR will play an increasingly valuable role in the analysis of plant nitrogen metabolism.

Citing Articles

Reversible Parahydrogen Induced Hyperpolarization of N in Unmodified Amino Acids Unraveled at High Magnetic Field.

Vaneeckhaute E, Tyburn J, Kempf J, Martens J, Breynaert E Adv Sci (Weinh). 2023; 10(23):e2207112.

PMID: 37211713 PMC: 10427394. DOI: 10.1002/advs.202207112.

Metabolomics and Transcriptomics Integration of Early Response of to Reduced Nitrogen Availability.

Chen M, Yin Y, Zhang L, Yang X, Fu T, Huo X Front Plant Sci. 2021; 12:769748.

PMID: 34956269 PMC: 8692568. DOI: 10.3389/fpls.2021.769748.

Comparative metabolic profiling of the lipid-producing green microalga reveals that nitrogen and carbon metabolic pathways contribute to lipid metabolism.

Chen H, Zheng Y, Zhan J, He C, Wang Q Biotechnol Biofuels. 2017; 10:153.

PMID: 28630648 PMC: 5471736. DOI: 10.1186/s13068-017-0839-4.

Understanding Plant Nitrogen Metabolism through Metabolomics and Computational Approaches.

Beatty P, Klein M, Fischer J, Lewis I, Muench D, Good A Plants (Basel). 2016; 5(4).

PMID: 27735856 PMC: 5198099. DOI: 10.3390/plants5040039.

Characterization of Early Enzymes Involved in TDP-Aminodideoxypentose Biosynthesis en Route to Indolocarbazole AT2433.

Peltier-Pain P, Singh S, Thorson J Chembiochem. 2015; 16(15):2141-6.

PMID: 26289554 PMC: 4598305. DOI: 10.1002/cbic.201500365.

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