Gene Expression Profiling in Single Cells Within Tissue

Overview

Journal Nat Methods

Specialties Biomedical Engineering
Pathology

Date 2005 Aug 25

PMID 16118636

Citations 28

Authors

Paola Capodieci

Michael Donovan

Heidi Buchinsky

Yusuf Jeffers

Carlos Cordon-Cardo

William Gerald

Jon Edelson

Shailesh M Shenoy

Robert H Singer

Affiliations

Soon will be listed here.

Abstract

We developed a robust multiplex fluorescent in situ hybridization (FISH) technique in archival formalin-fixed, paraffin-embedded (FFPE) human tissue sections while preserving the microanatomical context. This identifies single-cell gene expression patterns by probing multiple, unique nascent RNA transcripts and yields predictive quantitative gene expression signatures.

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Mapping a multiplexed zoo of mRNA expression.

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