Detection of MutA Genes in Transmitted Strains and Nontransmitted Strains of Mutans Streptococci

Our aim was to determine whether an isolate carrying one of the mutA genes was related to its ability to be transmitted from mother to her child. First, 200 mutans streptococci isolates were genotyped by arbitrarily primed polymerase chain reaction (AP-PCR) to demonstrate transmission between 20 mother-child pairs and to detect the transmitted and nontransmitted strains. Then the mutacin structural genes mutA encoding mutacin types I, II, and III were screened by PCR. The results showed that all strains found to carry the mutAI gene were nontransmitted strains; PCR screening primers mutAII and mutAIII did not yield amplicons in any of the strains tested. Our data suggest that an isolate carrying the mutAI gene is related to reduced transmission. The low frequency of detection of mutAII, and mutAIII suggests that there is a high heterogeneity in the genetic determinants needed for the production of mutacin-like substances.