Suitability of the PrfA Gene, Which Encodes a Regulator of Virulence Genes in Listeria Monocytogenes, in the Identification of Pathogenic Listeria Spp

Overview

Journal Appl Environ Microbiol

Specialties Environmental Health
Microbiology

Date 1992 Feb 1

PMID 1610204

Citations 18

Authors

K Wernars

K Heuvelman

S Notermans

E Domann

T Chakraborty

Affiliations

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Abstract

The pathogenesis of listerial infections is complex and involves a number of virulence factors expressed by virulent Listeria species. We have recently described a regulator gene, prfA, that positively regulates the expression of a number of virulence factors in Listeria monocytogenes. When the prfA gene was used as a DNA probe, we found it to be extremely specific for the pathogenic species L. monocytogenes. No reaction was obtained with strains of all other species of this genus. By using this information, an oligonucleotide primer pair was developed that specifically amplifies the prfA gene in L. monocytogenes strains of all known serotypes.

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