Genome Organization and Expression of the Penicillium Stoloniferum Virus F

Overview

Journal Virus Genes

Specialties Microbiology
Molecular Biology

Date 2005 Jul 19

PMID 16025243

Citations 19

Authors

Jong Wook Kim

Eun Young Choi

Jae Il Lee

Affiliations

Soon will be listed here.

Abstract

The complete sequences of three double-stranded (ds) RNAs (referred to F1, F2 and F3) of Penicillium stoloniferum virus F (PsV-F) were established. The F1 dsRNA was 1677 bp in length, and it contained one open reading frame (ORF) of 538 amino acids (molecular weight of 63 kDa, referred to P63), The F2 dsRNA was 1500 by in length, and also it contained one ORF of 420 amino acids (molecular weight of 46 kDa, referred to P46). The F3 dsRNA was 677 bp in length, but contained a small ORF with unknown function. A sequence motif of (5'-CGTAAAA-3') was found only at the 5' termini of the F1 and F2 dsRNAs, and a sequence motif of (5'-TAAAAAAAAA-3') was found at the 3' termini of all three dsRNA segments. The predicted amino acid sequence of F1 showed 38-48% sequence homology with the putative dsRNA-dependent RNA polymerases (RdRp) of dsRNA viruses, but the predicted amino acid of F2 showed no homology. Phylogenetic analysis using the RdRp sequences of the various Partitiviruses and Alphacryptoviruses revealed that PsV-F clustered well with Partitiviruses, but showed remote relationship with PsV-S. Near full-length and positive-sense single-stranded (ss) RNAs derived from the Fl, F2 and F3 dsRNAs were detected from the PsV-infected host cell. The expressed proteins of P63 and P46 showed a positive reaction against PsV-F antiserum, indicating P63 and P46 as RdRp and capsid protein, respectively. These results suggest that PsV-F can be a member of Partitivirus, but it is quite distinct from PsV-S electrophoretically, serologically and genetically, though both viruses coexist in the same cell.

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References

Still P, Detroy R, HESSELTINE C . Penicillium stoloniferum virus: altered replication in ultraviolet-derived mutants. J Gen Virol. 1975; 27(3):275-81. DOI: 10.1099/0022-1317-27-3-275. View

Bozarth R, Harley E . The electrophoretic mobility of double-stranded RNA in polyacrylamide gels as a function of molecular weight. Biochim Biophys Acta. 1976; 432(3):329-35. DOI: 10.1016/0005-2787(76)90142-8. View

Osaki H, Wei C, Arakawa M, Iwanami T, Nomura K, Matsumoto N . Nucleotide sequences of double-stranded RNA segments from a hypovirulent strain of the white root rot fungus Rosellinia necatrix: possibility of the first member of the Reoviridae from fungus. Virus Genes. 2002; 25(1):101-7. DOI: 10.1023/a:1020182427439. View

Bozarth R, Wood H, Mandelbrot A . The Penicillium stoloniferum virus complex: two similar double-stranded RNA virus-like particles in a single cell. Virology. 1971; 45(2):516-23. DOI: 10.1016/0042-6822(71)90352-7. View

Qian B, Kibenge F . Observations on polymerase chain reaction amplification of infectious bursal disease virus dsRNA. J Virol Methods. 1994; 47(1-2):237-42. DOI: 10.1016/0166-0934(94)90081-7. View

BAILEY J, Davidson N . Methylmercury as a reversible denaturing agent for agarose gel electrophoresis. Anal Biochem. 1976; 70(1):75-85. DOI: 10.1016/s0003-2697(76)80049-8. View

Compel P, Papp I, Bibo M, Fekete C, Hornok L . Genetic interrelationships and genome organization of double-stranded RNA elements of Fusarium poae. Virus Genes. 1999; 18(1):49-56. DOI: 10.1023/a:1008069318838. View

Tuomivirta T, Hantula J . Two unrelated double-stranded RNA molecule patterns in Gremmeniella abietina type A code for putative viruses of the families Totiviridae and Partitiviridae. Arch Virol. 2003; 148(12):2293-305. DOI: 10.1007/s00705-003-0194-6. View

Volloch V, Schweitzer B, Rits S . Ligation-mediated amplification of RNA from murine erythroid cells reveals a novel class of beta globin mRNA with an extended 5'-untranslated region. Nucleic Acids Res. 1994; 22(13):2507-11. PMC: 308202. DOI: 10.1093/nar/22.13.2507. View

10.

LHOAS P . Transmission of double stranded RNA viruses to a strain of Penicillium stoloniferum through heterokaryosis. Nature. 1971; 230(5291):248-9. DOI: 10.1038/230248a0. View

11.

Rong R, Rao S, Scott S, Carner G, Tainter F . Complete sequence of the genome of two dsRNA viruses from Discula destructiva. Virus Res. 2002; 90(1-2):217-24. DOI: 10.1016/s0168-1702(02)00178-8. View

12.

Poch O, Sauvaget I, Delarue M, Tordo N . Identification of four conserved motifs among the RNA-dependent polymerase encoding elements. EMBO J. 1989; 8(12):3867-74. PMC: 402075. DOI: 10.1002/j.1460-2075.1989.tb08565.x. View

13.

Osaki H, Kudo A, Ohtsu Y . Nucleotide sequence of seed- and pollen-transmitted double-stranded RNA, which encodes a putative RNA-dependent RNA polymerase, detected from Japanese pear. Biosci Biotechnol Biochem. 1999; 62(11):2101-6. DOI: 10.1271/bbb.62.2101. View

14.

Nogawa M, Kageyama T, Nakatani A, Taguchi G, Shimosaka M, Okazaki M . Cloning and characterization of mycovirus double-stranded RNA from the plant pathogenic fungus, Fusarium solani f. sp. robiniae. Biosci Biotechnol Biochem. 1996; 60(5):784-8. DOI: 10.1271/bbb.60.784. View

15.

Attoui H, Billoir F, Cantaloube J, Biagini P, De Micco P, de Lamballerie X . Strategies for the sequence determination of viral dsRNA genomes. J Virol Methods. 2000; 89(1-2):147-58. DOI: 10.1016/s0166-0934(00)00212-3. View

16.

England T, GUMPORT R, Uhlenbeck O . Dinucleoside pyrophosphate are substrates for T4-induced RNA ligase. Proc Natl Acad Sci U S A. 1977; 74(11):4839-42. PMC: 432051. DOI: 10.1073/pnas.74.11.4839. View

17.

Buck K . Biophysical properties of Penicillium stoloniferum virus S. J Gen Virol. 1973; 18(3):223-35. DOI: 10.1099/0022-1317-18-3-223. View

18.

Xie W, Antoniw J, White R . Nucleotide sequence of beet cryptic virus 3 dsRNA2 which encodes a putative RNA-dependent RNA polymerase. J Gen Virol. 1993; 74 ( Pt 7):1467-70. DOI: 10.1099/0022-1317-74-7-1467. View

19.

Thompson J, Higgins D, Gibson T . CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res. 1994; 22(22):4673-80. PMC: 308517. DOI: 10.1093/nar/22.22.4673. View

20.

STRAUSS E, Lakshman D, Tavantzis S . Molecular characterization of the genome of a partitivirus from the basidiomycete Rhizoctonia solani. J Gen Virol. 2000; 81(Pt 2):549-55. DOI: 10.1099/0022-1317-81-2-549. View