Expression of Albumin, IGF-1, IGFBP-3 in Tumor Tissues and Adjacent Non-tumor Tissues of Hepatocellular Carcinoma Patients with Cirrhosis

Overview

Journal World J Gastroenterol

Publisher Baishideng Publishing Group

Specialty Gastroenterology

Date 2005 Jul 15

PMID 16015705

Citations 23

Authors

Shi-min Luo

Wei-Min Tan

Wei-Xiong Deng

Si-min Zhuang

Jian-Wei Luo

Affiliations

Soon will be listed here.

Abstract

Aim: To explore the expression of albumin (ALB), insulin-like growth factor (IGF)-1, and insulin-like growth factor binding protein (IGFBP)-3 in tumor tissues and adjacent non-tumor tissues of hepatocellular carcinoma (HCC) patients with cirrhosis.

Methods: Twenty-four HCC patients with cirrhosis who underwent hepatectomy were studied. ALB mRNA, IGF-1 mRNA, and IGFBP-3 mRNA in liver tissues (including tumor tissues and adjacent non-tumor tissues) were detected by reverse transcriptase-polymerase chain reaction (RT-PCR). Liver Ki67 immunohistochemistry staining was studied. At the same time, 12 patients with cholelithiasis or liver angioma who underwent operation were segregated as normal control.

Results: In HCC patients with cirrhosis, hepatic ALB mRNA, IGF-1 mRNA, and IGFBP-3 mRNA of tumor tissues or adjacent non-tumor tissues were lower than the normal liver tissues, while in tumor tissues, hepatic ALB mRNA and IGFBP-3 mRNA were lower, hepatic IGF-1 mRNA was higher than in adjacent non-tumor tissues. Liver Ki67 labeling index (Ki67 LI) in tumor tissues or adjacent non-tumor tissues were higher than that in the normal liver tissues, while in tumor tissues it was higher than that in adjacent non-tumor tissues.

Conclusion: Imbalance of IGF-1 and IGFBP-3 may play a role in hepatocarcinogenesis and tumor development of liver cirrhosis patients.

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References

Rajaram S, Baylink D, Mohan S . Insulin-like growth factor-binding proteins in serum and other biological fluids: regulation and functions. Endocr Rev. 1997; 18(6):801-31. DOI: 10.1210/edrv.18.6.0321. View

Kuemmerle J, Murthy K, Bowers J . IGFBP-3 activates TGF-beta receptors and directly inhibits growth in human intestinal smooth muscle cells. Am J Physiol Gastrointest Liver Physiol. 2004; 287(4):G795-802. DOI: 10.1152/ajpgi.00009.2004. View

Gerdes J, Schwab U, Lemke H, STEIN H . Production of a mouse monoclonal antibody reactive with a human nuclear antigen associated with cell proliferation. Int J Cancer. 1983; 31(1):13-20. DOI: 10.1002/ijc.2910310104. View

Schoenle E, Zapf J, PRADER A, Torresani T, WERDER E, ZACHMANN M . Replacement of growth hormone (GH) in normally growing GH-deficient patients operated for craniopharyngioma. J Clin Endocrinol Metab. 1995; 80(2):374-8. DOI: 10.1210/jcem.80.2.7852493. View

Holly J, Wass J . Insulin-like growth factors; autocrine, paracrine or endocrine? New perspectives of the somatomedin hypothesis in the light of recent developments. J Endocrinol. 1989; 122(3):611-8. DOI: 10.1677/joe.0.1220611. View

Sidlova K, Pechova M, Kotaska K, Prusa R . Insulin-like growth factor binding protein-3 in patients with liver cirrhosis. Physiol Res. 2003; 51(6):587-90. View

Tanaka S, Takenaka K, Matsumata T, Mori R, Sugimachi K . Hepatitis C virus replication is associated with expression of transforming growth factor-alpha and insulin-like growth factor-II in cirrhotic livers. Dig Dis Sci. 1996; 41(1):208-15. DOI: 10.1007/BF02208606. View

ODell S, Day I . Insulin-like growth factor II (IGF-II). Int J Biochem Cell Biol. 1998; 30(7):767-71. DOI: 10.1016/s1357-2725(98)00048-x. View

Mathews L, Norstedt G, Palmiter R . Regulation of insulin-like growth factor I gene expression by growth hormone. Proc Natl Acad Sci U S A. 1986; 83(24):9343-7. PMC: 387134. DOI: 10.1073/pnas.83.24.9343. View

10.

Yamaguchi K, Nalesnik M, Carr B . In situ hybridization of albumin mRNA in normal liver and liver tumors: identification of hepatocellular origin. Virchows Arch B Cell Pathol Incl Mol Pathol. 1993; 64(6):361-5. DOI: 10.1007/BF02915135. View

11.

Murray C, Lopez A . Mortality by cause for eight regions of the world: Global Burden of Disease Study. Lancet. 1997; 349(9061):1269-76. DOI: 10.1016/S0140-6736(96)07493-4. View

12.

Vyzantiadis T, Theodoridou S, Giouleme O, Harsoulis P, Evgenidis N, Vyzantiadis A . Serum concentrations of insulin-like growth factor-I (IGF-I) in patients with liver cirrhosis. Hepatogastroenterology. 2003; 50(51):814-6. View

13.

Jerome L, Shiry L, Leyland-Jones B . Deregulation of the IGF axis in cancer: epidemiological evidence and potential therapeutic interventions. Endocr Relat Cancer. 2004; 10(4):561-78. DOI: 10.1677/erc.0.0100561. View

14.

Ozaki I, Motomura M, Setoguchi Y, Fujio N, Yamamoto K, Kariya T . Albumin mRNA expression in human liver diseases and its correlation to serum albumin concentration. Gastroenterol Jpn. 1991; 26(4):472-6. DOI: 10.1007/BF02782816. View

15.

Kang-Park S, Lee J, Shin J, Lee Y . Activation of the IGF-II gene by HBV-X protein requires PKC and p44/p42 map kinase signalings. Biochem Biophys Res Commun. 2001; 283(2):303-7. DOI: 10.1006/bbrc.2001.4767. View

16.

Sell S, Thomas K, Michalson M, Salatrepat J, Bonner J . Control of albumin and alpha-fetoprotein expression in rat liver and in some transplantable hepatocellular carcinomas. Biochim Biophys Acta. 1979; 564(1):173-8. DOI: 10.1016/0005-2787(79)90198-9. View

17.

Kratzsch J, Blum W, Schenker E, Keller E, Jahreis G, Haustein B . Measurement of insulin-like growth factor I (IGF-I) in normal adults, patients with liver cirrhosis and acromegaly: experience with a new competitive enzyme immunoassay. Exp Clin Endocrinol. 1993; 101(3):144-9. DOI: 10.1055/s-0029-1211221. View

18.

DErcole A, Stiles A, Underwood L . Tissue concentrations of somatomedin C: further evidence for multiple sites of synthesis and paracrine or autocrine mechanisms of action. Proc Natl Acad Sci U S A. 1984; 81(3):935-9. PMC: 344954. DOI: 10.1073/pnas.81.3.935. View

19.

Ali O, Cohen P, Lee K . Epidemiology and biology of insulin-like growth factor binding protein-3 (IGFBP-3) as an anti-cancer molecule. Horm Metab Res. 2004; 35(11-12):726-33. DOI: 10.1055/s-2004-814146. View

20.

Williams A, Collard T, Perks C, Newcomb P, Moorghen M, Holly J . Increased p53-dependent apoptosis by the insulin-like growth factor binding protein IGFBP-3 in human colonic adenoma-derived cells. Cancer Res. 2000; 60(1):22-7. View