Reversible Molecular Photoswitches: a Key Technology for Nanoscience and Fluorescence Imaging

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 2005 Jun 29

PMID 15983383

Citations 12

Authors

Markus Sauer

Affiliations

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Citing Articles

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Optical painting and fluorescence activated sorting of single adherent cells labelled with photoswitchable Pdots.

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Accounting for photophysical processes and specific signal intensity changes in fluorescence-detected sedimentation velocity.

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Optical control and study of biological processes at the single-cell level in a live organism.

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A unique series of reversibly switchable fluorescent proteins with beneficial properties for various applications.

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References

Verkhusha V, Lukyanov K . The molecular properties and applications of Anthozoa fluorescent proteins and chromoproteins. Nat Biotechnol. 2004; 22(3):289-96. DOI: 10.1038/nbt943. View

Habuchi S, Ando R, Dedecker P, Verheijen W, Mizuno H, Miyawaki A . Reversible single-molecule photoswitching in the GFP-like fluorescent protein Dronpa. Proc Natl Acad Sci U S A. 2005; 102(27):9511-6. PMC: 1157093. DOI: 10.1073/pnas.0500489102. View

Moresco F, Meyer G, Rieder K, Tang H, Gourdon A, Joachim C . Conformational changes of single molecules induced by scanning tunneling microscopy manipulation: a route to molecular switching. Phys Rev Lett. 2001; 86(4):672-5. DOI: 10.1103/PhysRevLett.86.672. View

Michalet X, Pinaud F, Bentolila L, Tsay J, Doose S, Li J . Quantum dots for live cells, in vivo imaging, and diagnostics. Science. 2005; 307(5709):538-44. PMC: 1201471. DOI: 10.1126/science.1104274. View

Fukaminato T, Sasaki T, Kawai T, Tamai N, Irie M . Digital photoswitching of fluorescence based on the photochromism of diarylethene derivatives at a single-molecule level. J Am Chem Soc. 2004; 126(45):14843-9. DOI: 10.1021/ja047169n. View

Tsien R . The green fluorescent protein. Annu Rev Biochem. 1998; 67:509-44. DOI: 10.1146/annurev.biochem.67.1.509. View

Betzig E, Chichester R . Single molecules observed by near-field scanning optical microscopy. Science. 1993; 262(5138):1422-5. DOI: 10.1126/science.262.5138.1422. View

Irie M . Diarylethenes for Memories and Switches. Chem Rev. 2002; 100(5):1685-1716. DOI: 10.1021/cr980069d. View

Dyba M, Hell S . Focal spots of size lambda/23 open up far-field fluorescence microscopy at 33 nm axial resolution. Phys Rev Lett. 2002; 88(16):163901. DOI: 10.1103/PhysRevLett.88.163901. View

10.

Tinnefeld P, Sauer M . Branching out of single-molecule fluorescence spectroscopy: challenges for chemistry and influence on biology. Angew Chem Int Ed Engl. 2005; 44(18):2642-2671. DOI: 10.1002/anie.200300647. View

11.

Dickson R, Cubitt A, Tsien R, Moerner W . On/off blinking and switching behaviour of single molecules of green fluorescent protein. Nature. 1997; 388(6640):355-8. DOI: 10.1038/41048. View

12.

Bates M, Blosser T, Zhuang X . Short-range spectroscopic ruler based on a single-molecule optical switch. Phys Rev Lett. 2005; 94(10):108101. PMC: 2652517. DOI: 10.1103/PhysRevLett.94.108101. View

13.

Bermudez V , CAPRON , Gase , GATTI , Kajzar , Leigh . Influencing intramolecular motion with an alternating electric field. Nature. 2000; 406(6796):608-11. DOI: 10.1038/35020531. View

14.

Chudakov D, Verkhusha V, Staroverov D, Souslova E, Lukyanov S, Lukyanov K . Photoswitchable cyan fluorescent protein for protein tracking. Nat Biotechnol. 2004; 22(11):1435-9. DOI: 10.1038/nbt1025. View

15.

Ando R, Mizuno H, Miyawaki A . Regulated fast nucleocytoplasmic shuttling observed by reversible protein highlighting. Science. 2004; 306(5700):1370-3. DOI: 10.1126/science.1102506. View

16.

Chattoraj M, King B, Bublitz G, Boxer S . Ultra-fast excited state dynamics in green fluorescent protein: multiple states and proton transfer. Proc Natl Acad Sci U S A. 1996; 93(16):8362-7. PMC: 38676. DOI: 10.1073/pnas.93.16.8362. View

17.

Klar T, Jakobs S, Dyba M, Egner A, Hell S . Fluorescence microscopy with diffraction resolution barrier broken by stimulated emission. Proc Natl Acad Sci U S A. 2000; 97(15):8206-10. PMC: 26924. DOI: 10.1073/pnas.97.15.8206. View

18.

Kennis J, Larsen D, van Stokkum I, Vengris M, van Thor J, van Grondelle R . Uncovering the hidden ground state of green fluorescent protein. Proc Natl Acad Sci U S A. 2004; 101(52):17988-93. PMC: 539731. DOI: 10.1073/pnas.0404262102. View

19.

Heilemann M, Margeat E, Kasper R, Sauer M, Tinnefeld P . Carbocyanine dyes as efficient reversible single-molecule optical switch. J Am Chem Soc. 2005; 127(11):3801-6. DOI: 10.1021/ja044686x. View

20.

Irie M, Fukaminato T, Sasaki T, Tamai N, Kawai T . Organic chemistry: a digital fluorescent molecular photoswitch. Nature. 2002; 420(6917):759-60. DOI: 10.1038/420759a. View