A Small Interfering RNA Targeting Coxsackievirus B3 Protects Permissive HeLa Cells from Viral Challenge

Overview

Journal J Virol

Publisher American Society for Microbiology

Date 2005 Jun 16

PMID 15956603

Citations 15

Authors

Jeonghyun Ahn

Eun Seok Jun

Hui Sun Lee

Seung Yong Yoon

Donghou Kim

Chul-Hyun Joo

Yoo Kyum Kim

Heuiran Lee

Affiliations

Soon will be listed here.

Abstract

We examined the ability of small interfering RNAs (siRNAs) to disrupt infection by coxsackievirus B3 (CVB3). The incorporation of siRNAs dramatically decreased cell death in permissive HeLa cells in parallel with a reduction in viral replication. Three of four siRNAs had potent anti-CVB3 activity. The present study thus demonstrates that the antiviral effect is due to the downregulation of viral replication. In addition, an effective CVB3-specific siRNA had similar antiviral effects in other related enteroviruses possessing sequence homology in the targeted region. Because the CVB3-specific siRNA is effective against other enteroviruses, siRNAs have potential for a universal anti-enterovirus strategy.

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References

Saksela K . Human viruses under attack by small inhibitory RNA. Trends Microbiol. 2003; 11(8):345-7. DOI: 10.1016/s0966-842x(03)00158-6. View

Dykxhoorn D, Novina C, Sharp P . Killing the messenger: short RNAs that silence gene expression. Nat Rev Mol Cell Biol. 2003; 4(6):457-67. DOI: 10.1038/nrm1129. View

Ahn J, Joo C, Seo I, Kim D, Hong H, Kim Y . Characteristics of apoptotic cell death induced by coxsackievirus B in permissive Vero cells. Intervirology. 2003; 46(4):245-51. DOI: 10.1159/000072435. View

Feuer R, Mena I, Pagarigan R, Harkins S, Hassett D, Whitton J . Coxsackievirus B3 and the neonatal CNS: the roles of stem cells, developing neurons, and apoptosis in infection, viral dissemination, and disease. Am J Pathol. 2003; 163(4):1379-93. PMC: 1868316. DOI: 10.1016/S0002-9440(10)63496-7. View

Khvorova A, Reynolds A, Jayasena S . Functional siRNAs and miRNAs exhibit strand bias. Cell. 2003; 115(2):209-16. DOI: 10.1016/s0092-8674(03)00801-8. View

Yoshinari K, Miyagishi M, Taira K . Effects on RNAi of the tight structure, sequence and position of the targeted region. Nucleic Acids Res. 2004; 32(2):691-9. PMC: 373345. DOI: 10.1093/nar/gkh221. View

Lingor P, Michel U, Scholl U, Bahr M, Kugler S . Transfection of "naked" siRNA results in endosomal uptake and metabolic impairment in cultured neurons. Biochem Biophys Res Commun. 2004; 315(4):1126-33. DOI: 10.1016/j.bbrc.2004.01.170. View

Reynolds A, Leake D, Boese Q, Scaringe S, Marshall W, Khvorova A . Rational siRNA design for RNA interference. Nat Biotechnol. 2004; 22(3):326-30. DOI: 10.1038/nbt936. View

Kanda T, Kusov Y, Yokosuka O, Gauss-Muller V . Interference of hepatitis A virus replication by small interfering RNAs. Biochem Biophys Res Commun. 2004; 318(2):341-5. DOI: 10.1016/j.bbrc.2004.03.194. View

10.

Ahn J, Choi J, Joo C, Seo I, Kim D, Yoon S . Susceptibility of mouse primary cortical neuronal cells to coxsackievirus B. J Gen Virol. 2004; 85(Pt 6):1555-1564. DOI: 10.1099/vir.0.19695-0. View

11.

Chen W, Yan W, Du Q, Fei L, Liu M, Ni Z . RNA interference targeting VP1 inhibits foot-and-mouth disease virus replication in BHK-21 cells and suckling mice. J Virol. 2004; 78(13):6900-7. PMC: 421660. DOI: 10.1128/JVI.78.13.6900-6907.2004. View

12.

Tracy S, Chapman N, Tu Z . Coxsackievirus B3 from an infectious cDNA copy of the genome is cardiovirulent in mice. Arch Virol. 1992; 122(3-4):399-409. DOI: 10.1007/BF01317202. View

13.

Muir P . Enteroviruses and heart disease. Br J Biomed Sci. 1993; 50(3):258-71. View

14.

Rotbart H . Enteroviral infections of the central nervous system. Clin Infect Dis. 1995; 20(4):971-81. DOI: 10.1093/clinids/20.4.971. View

15.

Tracy S, Chapman N, Romero J, Ramsingh A . Genetics of coxsackievirus B cardiovirulence and inflammatory heart muscle disease. Trends Microbiol. 1996; 4(5):175-9. DOI: 10.1016/0966-842x(96)10026-3. View

16.

Fire A, Xu S, Montgomery M, Kostas S, Driver S, Mello C . Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans. Nature. 1998; 391(6669):806-11. DOI: 10.1038/35888. View

17.

Rotbart H, Brennan P, Fife K, Romero J, Griffin J, McKinlay M . Enterovirus meningitis in adults. Clin Infect Dis. 1998; 27(4):896-8. DOI: 10.1086/517162. View

18.

Tomari Y, Matranga C, Haley B, Martinez N, Zamore P . A protein sensor for siRNA asymmetry. Science. 2004; 306(5700):1377-80. DOI: 10.1126/science.1102755. View

19.

Roivainen M . Enteroviruses and myocardial infarction. Am Heart J. 1999; 138(5 Pt 2):S479-83. DOI: 10.1016/s0002-8703(99)70280-2. View

20.

Huber S, Budd R, Rossner K, Newell M . Apoptosis in coxsackievirus B3-induced myocarditis and dilated cardiomyopathy. Ann N Y Acad Sci. 2000; 887:181-90. DOI: 10.1111/j.1749-6632.1999.tb07932.x. View