Biological Effects of Helium-neon Laser Irradiation on Normal and Wounded Human Skin Fibroblasts

Overview

Journal Photomed Laser Surg

Specialty General Surgery

Date 2005 Jun 16

PMID 15954811

Citations 24

Authors

D Hawkins

H Abrahamse

Affiliations

Soon will be listed here.

Abstract

Objective: This study aimed to investigate a number of structural, cellular, and molecular responses to heliumneon (632.8 nm) laser irradiation following a single dose of 0.5, 2.5, 5, or 10 J/cm2 on normal and wounded human skin fibroblasts.

Background Data: Low-level laser therapy (LLLT) is a form of phototherapy, involving the application of low-power monochromatic and coherent light to injuries and lesions to stimulate healing. 1 This therapy has been successfully used for pain attenuation and to induce wound healing in nonhealing defects.

2 Methods: Changes in normal and wounded fibroblast cell morphology were evaluated by light microscopy. Cellular parameters evaluated cell proliferation, cell viability, and cytotoxicity while molecular parameters assessed the extent of DNA damage.

Results: The results clearly demonstrate that LLLT has an effect on normal and wounded(3) human skin fibroblasts. The parameters showed that doses of 0.5, 2.5, 5, and 10 J/cm2 were sufficient to produce measurable changes in fibroblast cells.

Conclusion: A dose of 10 J/cm2 appeared to produce a significant amount of cellular and molecular damage, which could be an important consideration for other therapies, such as photodynamic therapy.

Citing Articles

Effect of LASER therapy on plantar fasciitis pain: illuminating a promising treatment approach - a systematic review.

Yadav S, Sharma S, Chatterjee S, Sharma A, Thakur S Lasers Med Sci. 2025; 40(1):18.

PMID: 39809999 DOI: 10.1007/s10103-025-04289-y.

The effect of combined red, blue, and near-infrared light-emitting diode (LED) photobiomodulation therapy on speed of wound healing after superficial ablative fractional resurfacing.

Soliman J, Elsanadi R, Messele F, Kelly K Lasers Med Sci. 2024; 39(1):94.

PMID: 38532146 PMC: 10965566. DOI: 10.1007/s10103-024-04042-x.

Optimal Parameters of Laser Therapy to Improve Critical Calvarial Defects.

Santos M, Silva D, Rovaris K, Sousa F, Dantas E, Loureiro L Front Physiol. 2022; 13:841146.

PMID: 35283760 PMC: 8914104. DOI: 10.3389/fphys.2022.841146.

A Critical Review on Selected External Physical Cues and Modulation of Cell Behavior: Magnetic Nanoparticles, Non-thermal Plasma and Lasers.

Smolkova B, Uzhytchak M, Lynnyk A, Kubinova S, Dejneka A, Lunov O J Funct Biomater. 2018; 10(1).

PMID: 30586923 PMC: 6463085. DOI: 10.3390/jfb10010002.

Photobiomodulation Therapy in the Treatment of Oral Mucositis, Dysgeusia and Oral Dryness as Side-Effects of Head and Neck Radiotherapy in a Cancer Patient: A Case Report.

El Mobadder M, Farhat F, El Mobadder W, Nammour S Dent J (Basel). 2018; 6(4).

PMID: 30423851 PMC: 6313426. DOI: 10.3390/dj6040064.