Identification of Coexpressed Gene Clusters in a Comparative Analysis of Transcriptome and Proteome in Mouse Tissues

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 2005 Jun 9

PMID 15939889

Citations 35

Authors

T Mijalski

A Harder

T Halder

M Kersten

M Horsch

T M Strom

H V Liebscher

F Lottspeich

M Hrabe de Angelis

J Beckers

Affiliations

Soon will be listed here.

Abstract

A major advantage of the mouse model lies in the increasing information on its genome, transcriptome, and proteome, as well as in the availability of a fast growing number of targeted and induced mutant alleles. However, data from comparative transcriptome and proteome analyses in this model organism are very limited. We use DNA chip-based RNA expression profiling and 2D gel electrophoresis, combined with peptide mass fingerprinting of liver and kidney, to explore the feasibility of such comprehensive gene expression analyses. Although protein analyses mostly identify known metabolic enzymes and structural proteins, transcriptome analyses reveal the differential expression of functionally diverse and not yet described genes. The comparative analysis suggests correlation between transcriptional and translational expression for the majority of genes. Significant exceptions from this correlation confirm the complementarities of both approaches. Based on RNA expression data from the 200 most differentially expressed genes, we identify chromosomal colocalization of known, as well as not yet described, gene clusters. The determination of 29 such clusters may suggest that coexpression of colocalizing genes is probably rather common.

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