Structural Basis of Chaperone-subunit Complex Recognition by the Type 1 Pilus Assembly Platform FimD

Overview

Journal EMBO J

Specialties Biotechnology
Molecular Biology

Date 2005 May 28

PMID 15920478

Citations 49

Authors

Mireille Nishiyama

Reto Horst

Oliv Eidam

Torsten Herrmann

Oleksandr Ignatov

Michael Vetsch

Pascal Bettendorff

Ilian Jelesarov

Markus G Grutter

Kurt Wuthrich

Rudi Glockshuber

Guido Capitani

Affiliations

Soon will be listed here.

Abstract

Adhesive type 1 pili from uropathogenic Escherichia coli are filamentous protein complexes that are attached to the assembly platform FimD in the outer membrane. During pilus assembly, FimD binds complexes between the chaperone FimC and type 1 pilus subunits in the periplasm and mediates subunit translocation to the cell surface. Here we report nuclear magnetic resonance and X-ray protein structures of the N-terminal substrate recognition domain of FimD (FimD(N)) before and after binding of a chaperone-subunit complex. FimD(N) consists of a flexible N-terminal segment of 24 residues, a structured core with a novel fold, and a C-terminal hinge segment. In the ternary complex, residues 1-24 of FimD(N) specifically interact with both FimC and the subunit, acting as a sensor for loaded FimC molecules. Together with in vivo complementation studies, we show how this mechanism enables recognition and discrimination of different chaperone-subunit complexes by bacterial pilus assembly platforms.

Citing Articles

Structural basis for adhesin secretion by the outer-membrane usher in type 1 pili.

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Stochastic chain termination in bacterial pilus assembly.

Giese C, Puorger C, Ignatov O, Becarova Z, Weber M, Scharer M Nat Commun. 2023; 14(1):7718.

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Archaic chaperone-usher pili self-secrete into superelastic zigzag springs.

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Processive dynamics of the usher assembly platform during uropathogenic Escherichia coli P pilus biogenesis.

Du M, Yuan Z, Werneburg G, Henderson N, Chauhan H, Kovach A Nat Commun. 2021; 12(1):5207.

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Reciprocal Packaging of the Main Structural Proteins of Type 1 Fimbriae and Flagella in the Outer Membrane Vesicles of "Wild Type" Strains.

Blackburn S, Shepherd M, Robinson G Front Microbiol. 2021; 12:557455.

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