Potentiation of Sympathetic Neuromuscular Transmission Mediated by Muscarinic Receptors in Guinea Pig Isolated Vas Deferens

Overview

Journal Naunyn Schmiedebergs Arch Pharmacol

Specialty Pharmacology

Date 2005 May 19

PMID 15900515

Citations 3

Authors

Samera Iram

Charles H V Hoyle

Affiliations

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Abstract

In guinea-pig isolated vasa deferentia, purinergic neurogenic contractions and responses to applied adenosine 5'-triphosphate (ATP) were potentiated by carbachol; responses to adrenergic transmission and applied noradrenaline were not. Following blockade of P2 receptors and alpha-adrenoceptors, the residual neurogenic response was massively potentiated by carbachol, suggesting the presence of a non-purinergic, non-adrenergic component. In the presence of guanethidine, carbachol had no significant effect, indicating that sympathetic transmission was the only element involved. Use of oxotremorine and selective muscarinic receptor antagonists suggested that the potentiating effect of carbachol and oxotremorine was mediated via M3 muscarinic receptors without involvement of nicotinic receptors.

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References

Barlow R, Shepherd M . A further search for selective antagonists at M2-muscarinic receptors. Br J Pharmacol. 1986; 89(4):837-43. PMC: 1917221. DOI: 10.1111/j.1476-5381.1986.tb11189.x. View

McLaren G, Lambrecht G, Mutschler E, Baumert H, Sneddon P, Kennedy C . Investigation of the actions of PPADS, a novel P2x-purinoceptor antagonist, in the guinea-pig isolated vas deferens. Br J Pharmacol. 1994; 111(3):913-7. PMC: 1910078. DOI: 10.1111/j.1476-5381.1994.tb14825.x. View

SJOSTRAND N . Effects of acetylcholine and some other smooth muscle stimulants on the electrical and mechanical responses of the guinea-pig vas deferens to nerve stimulation. Acta Physiol Scand. 1973; 89(1):1-9. DOI: 10.1111/j.1748-1716.1973.tb05491.x. View

Hammer R, Berrie C, Birdsall N, BURGEN A, Hulme E . Pirenzepine distinguishes between different subclasses of muscarinic receptors. Nature. 1980; 283(5742):90-2. DOI: 10.1038/283090a0. View

Caulfield M, Birdsall N . International Union of Pharmacology. XVII. Classification of muscarinic acetylcholine receptors. Pharmacol Rev. 1998; 50(2):279-90. View

Ellis J, Burnstock G . Neuropeptide Y neuromodulation of sympathetic co-transmission in the guinea-pig vas deferens. Br J Pharmacol. 1990; 100(3):457-62. PMC: 1917766. DOI: 10.1111/j.1476-5381.1990.tb15828.x. View

Ellis J, Burnstock G . Modulation of neurotransmission in the guinea-pig vas deferens by capsaicin: involvement of calcitonin gene-related peptide and substance P. Br J Pharmacol. 1989; 98(2):707-13. PMC: 1854699. DOI: 10.1111/j.1476-5381.1989.tb12646.x. View

Hernandez M, Simonsen U, Prieto D, Rivera L, Garcia P, Ordaz E . Different muscarinic receptor subtypes mediating the phasic activity and basal tone of pig isolated intravesical ureter. Br J Pharmacol. 1993; 110(4):1413-20. PMC: 2175837. DOI: 10.1111/j.1476-5381.1993.tb13978.x. View

Lundberg J, Stjarne L . Neuropeptide Y (NPY) depresses the secretion of 3H-noradrenaline and the contractile response evoked by field stimulation, in rat vas deferens. Acta Physiol Scand. 1984; 120(3):477-9. DOI: 10.1111/j.1748-1716.1984.tb07410.x. View

10.

Song Z, Brookes S, Gibbins I, Costa M . NADPH-diaphorase and other neuronal markers in nerves and ganglia supplying the guinea-pig vas deferens. J Auton Nerv Syst. 1994; 48(1):31-43. DOI: 10.1016/0165-1838(94)90157-0. View

11.

Hammer R, Giachetti A . Muscarinic receptor subtypes: M1 and M2 biochemical and functional characterization. Life Sci. 1982; 31(26):2991-8. DOI: 10.1016/0024-3205(82)90066-2. View

12.

von Kugelgen I, Starke K . Release of noradrenaline and ATP by electrical stimulation and nicotine in guinea-pig vas deferens. Naunyn Schmiedebergs Arch Pharmacol. 1991; 344(4):419-29. DOI: 10.1007/BF00172581. View

13.

Michel A, Stefanich E, Whiting R . Direct labeling of rat M3-muscarinic receptors by [3H]4DAMP. Eur J Pharmacol. 1989; 166(3):459-66. DOI: 10.1016/0014-2999(89)90359-2. View

14.

Stjernquist M, Owman C, SJOBERG N, Sundler F . Coexistence and cooperation between neuropeptide Y and norepinephrine in nerve fibers of guinea pig vas deferens and seminal vesicle. Biol Reprod. 1987; 36(1):149-55. DOI: 10.1095/biolreprod36.1.149. View

15.

Eltze M . Muscarinic M1- and M2-receptors mediating opposite effects on neuromuscular transmission in rabbit vas deferens. Eur J Pharmacol. 1988; 151(2):205-21. DOI: 10.1016/0014-2999(88)90801-1. View

16.

Kasakov L, Burnstock G . The use of the slowly degradable analog, alpha, beta-methylene ATP, to produce desensitisation of the P2-purinoceptor: effect on non-adrenergic, non-cholinergic responses of the guinea-pig urinary bladder. Eur J Pharmacol. 1982; 86(2):291-4. DOI: 10.1016/0014-2999(82)90330-2. View

17.

Lee C . Potentiation by cholinoceptor agonists of contractions to field stimulation of rat vas deferens. Br J Pharmacol. 1985; 86(3):671-6. PMC: 1916734. DOI: 10.1111/j.1476-5381.1985.tb08944.x. View

18.

Lambrecht G, Friebe T, Grimm U, Windscheif U, Bungardt E, Hildebrandt C . PPADS, a novel functionally selective antagonist of P2 purinoceptor-mediated responses. Eur J Pharmacol. 1992; 217(2-3):217-9. DOI: 10.1016/0014-2999(92)90877-7. View

19.

Meldrum L, Burnstock G . Evidence that ATP acts as a co-transmitter with noradrenaline in sympathetic nerves supplying the guinea-pig vas deferens. Eur J Pharmacol. 1983; 92(1-2):161-3. DOI: 10.1016/0014-2999(83)90126-7. View

20.

Matsuno K, Mita S . Involvement of the muscarinic receptors in the postsynaptic potentiation of neurogenic twitch contraction in the mouse vas deferens. Life Sci. 1992; 50(11):799-806. DOI: 10.1016/0024-3205(92)90185-r. View