Vitreous Collagen Metabolism Before and After Vitrectomy

Overview

Journal Graefes Arch Clin Exp Ophthalmol

Specialty Ophthalmology

Date 2005 May 19

PMID 15900480

Citations 4

Authors

Hirotaka Itakura

Shoji Kishi

Nobuo Kotajima

Masami Murakami

Affiliations

Soon will be listed here.

Abstract

Purpose: To assess vitreous metabolism by measuring C-propeptide levels of type II procollagen (pCOL-II-C) and hyaluronan levels in the vitreous and in the vitreous fluid after vitrectomy for macular hole.

Methods: We obtained 1-ml vitreous samples during vitrectomy from 34 patients (34 eyes) with a macular hole (age range 50-77 years, mean 64 years). After vitrectomy, we performed fluid-air exchange in six eyes because of unresolved macular holes and collected 4-ml fluid samples. Gel-filtration high-performance liquid chromatography (HPLC) was used to determine the molecular weight of pCOL-II-C in the samples. The pCOL-II-C level was measured by sandwich enzyme immunoassay and hyaluronan by sandwich binding protein assay.

Results: HPLC showed that pCOL-II-C in the vitreous samples corresponded to purified pCOL-II-C from cartilage. The vitreous pCOL-II-C level (4.7+/-0.3 ng/ml) was similar to reported synovial fluid levels. In six eyes that underwent fluid-air exchange, pCOL-II-C in the fluid samples remained at a level similar to that in the vitreous samples, while hyaluronan levels in the fluid samples were significantly lower than in the vitreous samples.

Conclusions: The molecular weight and concentrations of pCOL-II-C in the vitreous are similar to those in joint fluid. In patients with a macular hole, type II procollagen may be secreted persistently into the vitreous cavity before and after vitrectomy.

Citing Articles

Evaluation of corneal hysteresis after pars plana vitrectomy combined phacoemulsification and intraocular lens implantation.

Ohta M, Wakuta M, Sakuma A, Hasegawa M, Hamada W, Higashijima F Sci Rep. 2022; 12(1):14630.

PMID: 36028519 PMC: 9418308. DOI: 10.1038/s41598-022-18299-1.

Diabetic macular edema treatment guidelines in India: All India Ophthalmological Society Diabetic Retinopathy Task Force and Vitreoretinal Society of India consensus statement.

Giridhar S, Verma L, Rajendran A, Bhende M, Goyal M, Ramasamy K Indian J Ophthalmol. 2021; 69(11):3076-3086.

PMID: 34708746 PMC: 8725123. DOI: 10.4103/ijo.IJO_1469_21.

Diffusion characteristics of vitreous humour and saline solution follow the Stokes Einstein equation.

Gisladottir S, Loftsson T, Stefansson E Graefes Arch Clin Exp Ophthalmol. 2009; 247(12):1677-84.

PMID: 19639333 DOI: 10.1007/s00417-009-1141-3.

Human retinal Müller cells synthesize collagens of the vitreous and vitreoretinal interface in vitro.

Ponsioen T, van Luyn M, Van der Worp R, Pas H, Hooymans J, Los L Mol Vis. 2008; 14:652-60.

PMID: 18385800 PMC: 2276182.

References

Takanosu M, Boyd T, Le Goff M, Henry S, Zhang Y, Bishop P . Structure, chromosomal location, and tissue-specific expression of the mouse opticin gene. Invest Ophthalmol Vis Sci. 2001; 42(10):2202-10. View

Shinmei M, Ito K, Matsuyama S, Yoshihara Y, Matsuzawa K . Joint fluid carboxy-terminal type II procollagen peptide as a marker of cartilage collagen biosynthesis. Osteoarthritis Cartilage. 1993; 1(2):121-8. DOI: 10.1016/s1063-4584(05)80027-5. View

Ihanamaki T, Salminen H, Saamanen A, Pelliniemi L, Hartmann D, Vuorio E . Age-dependent changes in the expression of matrix components in the mouse eye. Exp Eye Res. 2001; 72(4):423-31. DOI: 10.1006/exer.2000.0972. View

Richards A, Morgan J, Bearcroft P, Pickering E, Owen M, Holmans P . Vitreoretinopathy with phalangeal epiphyseal dysplasia, a type II collagenopathy resulting from a novel mutation in the C-propeptide region of the molecule. J Med Genet. 2002; 39(9):661-5. PMC: 1735224. DOI: 10.1136/jmg.39.9.661. View

Reardon A, Sandell L, Jones C, McLeod D, Bishop P . Localization of pN-type IIA procollagen on adult bovine vitreous collagen fibrils. Matrix Biol. 2000; 19(2):169-73. DOI: 10.1016/s0945-053x(00)00058-5. View

Bishop P, Takanosu M, Le Goff M, Mayne R . The role of the posterior ciliary body in the biosynthesis of vitreous humour. Eye (Lond). 2002; 16(4):454-60. DOI: 10.1038/sj.eye.6700199. View

Nelson F, Dahlberg L, Laverty S, Reiner A, Pidoux I, Ionescu M . Evidence for altered synthesis of type II collagen in patients with osteoarthritis. J Clin Invest. 1998; 102(12):2115-25. PMC: 509166. DOI: 10.1172/JCI4853. View

Savontaus M, Ihanamaki T, Metsaranta M, Vuorio E . Localization of type II collagen mRNA isoforms in the developing eyes of normal and transgenic mice with a mutation in type II collagen gene. Invest Ophthalmol Vis Sci. 1997; 38(5):930-42. View

Carey D, Alini M, Ionescu M, Hyams J, Rowe J, Rosenberg L . Serum content of the C-propeptide of the cartilage molecule type II collagen in children. Clin Exp Rheumatol. 1997; 15(3):325-8. View

10.

Bishop P, Crossman M, McLeod D, Ayad S . Extraction and characterization of the tissue forms of collagen types II and IX from bovine vitreous. Biochem J. 1994; 299 ( Pt 2):497-505. PMC: 1138299. DOI: 10.1042/bj2990497. View

11.

Bishop P, Reardon A, McLeod D, Ayad S . Identification of alternatively spliced variants of type II procollagen in vitreous. Biochem Biophys Res Commun. 1994; 203(1):289-95. DOI: 10.1006/bbrc.1994.2180. View

12.

Chichibu K, Matsuura T, Shichijo S, Yokoyama M . Assay of serum hyaluronic acid in clinical application. Clin Chim Acta. 1989; 181(3):317-23. DOI: 10.1016/0009-8981(89)90237-4. View

13.

Laurent T, Fraser J . The properties and turnover of hyaluronan. Ciba Found Symp. 1986; 124:9-29. DOI: 10.1002/9780470513385.ch2. View

14.

Larsson L, OSTERLIN S . Posterior vitreous detachment. A combined clinical and physiochemical study. Graefes Arch Clin Exp Ophthalmol. 1985; 223(2):92-5. DOI: 10.1007/BF02150952. View

15.

Bishop P . Structural macromolecules and supramolecular organisation of the vitreous gel. Prog Retin Eye Res. 2000; 19(3):323-44. DOI: 10.1016/s1350-9462(99)00016-6. View

16.

Sugiyama S, Itokazu M, Suzuki Y, Shimizu K . Procollagen II C propeptide level in the synovial fluid as a predictor of radiographic progression in early knee osteoarthritis. Ann Rheum Dis. 2002; 62(1):27-32. PMC: 1754298. DOI: 10.1136/ard.62.1.27. View

17.

Atkinson E, Jones S, Ellis B, Dumonde D, Graham E . Molecular size of retinal vascular leakage determined by FITC-dextran angiography in patients with posterior uveitis. Eye (Lond). 1991; 5 ( Pt 4):440-6. DOI: 10.1038/eye.1991.71. View

18.

Uitto J, Allan R, Polak K . Conversion of type II procollagen to collagen. Extracellular removal of the amino-terminal and carboxy-terminal extensions without a preferential sequence. Eur J Biochem. 1979; 99(1):97-103. DOI: 10.1111/j.1432-1033.1979.tb13236.x. View

19.

Rebuck N, Croucher L, Hollander A . Distribution of two alternatively spliced variants of the type II collagen N-propeptide compared with the C-propeptide in bovine chondrocyte pellet cultures. J Cell Biochem. 1999; 75(1):13-21. DOI: 10.1002/(sici)1097-4644(19991001)75:1<13::aid-jcb2>3.3.co;2-z. View

20.

Newsome D, Linsenmayer T, Trelstad R . Vitreous body collagen. Evidence for a dual origin from the neural retina and hyalocytes. J Cell Biol. 1976; 71(1):59-67. PMC: 2109724. DOI: 10.1083/jcb.71.1.59. View