Interaction Cloning: Identification of a Helix-loop-helix Zipper Protein That Interacts with C-Fos

Overview

Journal Science

Specialty Science

Date 1992 May 15

PMID 1589769

Citations 119

Authors

M A Blanar

W J Rutter

Affiliations

Soon will be listed here.

Abstract

A facile method for isolating genes that encode interacting proteins has been developed with a polypeptide probe that contains an amino-terminal extension with recognition sites for a monoclonal antibody, a specific endopeptidase, and a site-specific protein kinase. This probe, containing the basic region-leucine zipper dimerization motif of c-Fos, was used to screen a complementary DNA library. A complementary DNA that encoded a member of the basic-helix-loop-helix-zipper (bHLH-Zip) family of proteins was isolated. The complementary DNA-encoded polypeptide FIP (Fos interacting protein) bound to oligonucleotide probes that contained DNA binding motifs for other HLH proteins. When cotransfected with c-Fos, FIP stimulated transcription of an AP-1-responsive promoter.

Citing Articles

USF2-mediated upregulation of TXNRD1 contributes to hepatocellular carcinoma progression by activating Akt/mTOR signaling.

Huang W, Liao Z, Zhang J, Zhang X, Zhang H, Liang H Cell Death Dis. 2022; 13(11):917.

PMID: 36319631 PMC: 9626593. DOI: 10.1038/s41419-022-05363-x.

Characterization of an anti-FLAG antibody binding protein in V. cholerae.

Shin J, Lanz M, Smolka M, Dorr T Biochem Biophys Res Commun. 2020; 528(3):493-498.

PMID: 32505345 PMC: 7357423. DOI: 10.1016/j.bbrc.2020.05.169.

Integrating Peak Colocalization and Motif Enrichment Analysis for the Discovery of Genome-Wide Regulatory Modules and Transcription Factor Recruitment Rules.

Ronzio M, Zambelli F, Dolfini D, Mantovani R, Pavesi G Front Genet. 2020; 11:72.

PMID: 32153638 PMC: 7046753. DOI: 10.3389/fgene.2020.00072.

Islet expression of Rhombotin and Isl-1 suggests cell type specific exposure of LIM-domain epitopes.

Lund K, Petersen J, Jensen J, Blume N, Edlund T, Thor S Endocrine. 2010; 3(6):399-408.

PMID: 21153242 DOI: 10.1007/BF02935644.

Purification and identification of a transcription factor, USF-2, binding to E-box element in the promoter of human telomerase reverse transcriptase (hTERT).

Jiang S, Galindo M, Jarrett H Proteomics. 2009; 10(2):203-11.

PMID: 19899074 PMC: 2851252. DOI: 10.1002/pmic.200800693.