Decreased Transcription of the Human FCGR2B Gene Mediated by the -343 G/C Promoter Polymorphism and Association with Systemic Lupus Erythematosus

Overview

Journal Hum Genet

Specialty Genetics

Date 2005 May 17

PMID 15895258

Citations 72

Authors

Marissa C Blank

Radu N Stefanescu

Emi Masuda

Francesc Marti

Philip D King

Patricia B Redecha

Robert J Wurzburger

Margaret G E Peterson

Shizuko Tanaka

Luminita Pricop

Affiliations

Soon will be listed here.

Abstract

The role for inhibitory Fc gamma receptors class IIb (FcgammaRIIb) in the onset, progression and severity of several animal models of autoimmune diseases is well established. By contrast, the pathogenic potential of FcgammaRIIb in human autoimmune diseases remains largely unknown. Here we report the identification of a polymorphism in the human FCGR2B promoter (dbSNP no. rs3219018) that is associated in homozygosity with systemic lupus erythematosus (SLE) phenotype in European-Americans (OR=11.1, P=0.003). Experimental evidence correlates the polymorphism (a G-C substitution at position -343 relative to the start of transcription) with altered FcgammaRIIb expression and function. The G-C substitution correlated with decreased transcription of the FCGR2B promoter, and resulted in decreased binding of the AP1 transcription complex to the mutant promoter sequence. The surface expression of FcgammaRIIb receptors was significantly reduced in activated B cells from (-343 C/C) SLE patients. These findings suggest that genetic defects may lead to deregulated expression of the FCGR2B gene in -343 C/C homozygous subjects, and may play a role in the pathogenesis of human SLE.

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