The Retinoblastoma Gene Product Regulates Sp1-mediated Transcription

Overview

Journal Mol Cell Biol

Specialty Cell Biology

Date 1992 Jun 1

PMID 1588949

Citations 65

Authors

S J Kim

U S Onwuta

Y I Lee

R Li

M R Botchan

P D Robbins

Affiliations

Soon will be listed here.

Abstract

We have demonstrated that the retinoblastoma gene product (Rb) can positively regulate transcription from the fourth promoter of the insulinlike growth factor II gene. Two copies of a motif (the retinoblastoma control element) similar to that found in the human c-fos, transforming growth factor beta 1, and c-myc promoters are responsible for conferring Rb regulation to the fourth promoter of the insulinlike growth factor II gene. We have shown that the transcription factor Sp1 can bind to and stimulate transcription from the retinoblastoma control element motif. Moreover, by using a GAL4-Sp1 fusion protein, we have directly demonstrated that Rb positively regulates Sp1 transcriptional activity in vivo. These results indicate that Rb can function as a positive regulator of transcription and that Sp1 is one potential target, either directly or indirectly, for transcriptional regulation by Rb.

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References

Fung Y, Murphree A, Tang A, Qian J, Hinrichs S, Benedict W . Structural evidence for the authenticity of the human retinoblastoma gene. Science. 1987; 236(4809):1657-61. DOI: 10.1126/science.2885916. View

Bookstein R, Shew J, Chen P, Scully P, Lee W . Suppression of tumorigenicity of human prostate carcinoma cells by replacing a mutated RB gene. Science. 1990; 247(4943):712-5. DOI: 10.1126/science.2300823. View

Lee E, To H, Shew J, Bookstein R, Scully P, Lee W . Inactivation of the retinoblastoma susceptibility gene in human breast cancers. Science. 1988; 241(4862):218-21. DOI: 10.1126/science.3388033. View

Kadonaga J, Carner K, Masiarz F, Tjian R . Isolation of cDNA encoding transcription factor Sp1 and functional analysis of the DNA binding domain. Cell. 1987; 51(6):1079-90. DOI: 10.1016/0092-8674(87)90594-0. View

Friend S, Horowitz J, Gerber M, Wang X, BOGENMANN E, Li F . Deletions of a DNA sequence in retinoblastomas and mesenchymal tumors: organization of the sequence and its encoded protein. Proc Natl Acad Sci U S A. 1987; 84(24):9059-63. PMC: 299691. DOI: 10.1073/pnas.84.24.9059. View

Lee W, Bookstein R, Hong F, Young L, Shew J, Lee E . Human retinoblastoma susceptibility gene: cloning, identification, and sequence. Science. 1987; 235(4794):1394-9. DOI: 10.1126/science.3823889. View

Kadonaga J, Courey A, Ladika J, Tjian R . Distinct regions of Sp1 modulate DNA binding and transcriptional activation. Science. 1988; 242(4885):1566-70. DOI: 10.1126/science.3059495. View

Horowitz J, Yandell D, Park S, Canning S, Whyte P, Buchkovich K . Point mutational inactivation of the retinoblastoma antioncogene. Science. 1989; 243(4893):937-40. DOI: 10.1126/science.2521957. View

Rustgi A, Dyson N, Bernards R . Amino-terminal domains of c-myc and N-myc proteins mediate binding to the retinoblastoma gene product. Nature. 1991; 352(6335):541-4. DOI: 10.1038/352541a0. View

10.

Chittenden T, Livingston D, Kaelin Jr W . The T/E1A-binding domain of the retinoblastoma product can interact selectively with a sequence-specific DNA-binding protein. Cell. 1991; 65(6):1073-82. DOI: 10.1016/0092-8674(91)90559-h. View

11.

Kaelin Jr W, Pallas D, DeCaprio J, Kaye F, Livingston D . Identification of cellular proteins that can interact specifically with the T/E1A-binding region of the retinoblastoma gene product. Cell. 1991; 64(3):521-32. DOI: 10.1016/0092-8674(91)90236-r. View

12.

Li R, Knight J, Jackson S, Tjian R, Botchan M . Direct interaction between Sp1 and the BPV enhancer E2 protein mediates synergistic activation of transcription. Cell. 1991; 65(3):493-505. DOI: 10.1016/0092-8674(91)90467-d. View

13.

Bandara L, Adamczewski J, Hunt T, La Thangue N . Cyclin A and the retinoblastoma gene product complex with a common transcription factor. Nature. 1991; 352(6332):249-51. DOI: 10.1038/352249a0. View

14.

Bagchi S, Weinmann R, Raychaudhuri P . The retinoblastoma protein copurifies with E2F-I, an E1A-regulated inhibitor of the transcription factor E2F. Cell. 1991; 65(6):1063-72. DOI: 10.1016/0092-8674(91)90558-g. View

15.

Chellappan S, Hiebert S, Mudryj M, Horowitz J, Nevins J . The E2F transcription factor is a cellular target for the RB protein. Cell. 1991; 65(6):1053-61. DOI: 10.1016/0092-8674(91)90557-f. View

16.

Marx J . The cell cycle: spinning farther afield. Science. 1991; 252(5012):1490-2. DOI: 10.1126/science.1828620. View

17.

Kim S, Lee H, Robbins P, Busam K, Sporn M, Roberts A . Regulation of transforming growth factor beta 1 gene expression by the product of the retinoblastoma-susceptibility gene. Proc Natl Acad Sci U S A. 1991; 88(8):3052-6. PMC: 51382. DOI: 10.1073/pnas.88.8.3052. View

18.

Huang P, Jones R, Haskell K, Vuocolo G, Hanobik M, Huber H . Cloning of cDNAs for cellular proteins that bind to the retinoblastoma gene product. Nature. 1991; 352(6332):251-4. DOI: 10.1038/352251a0. View

19.

Wagner S, Green M . Retinoblastoma. A transcriptional tryst. Nature. 1991; 352(6332):189-90. DOI: 10.1038/352189a0. View

20.

Huang S, Lee W, Lee E . A cellular protein that competes with SV40 T antigen for binding to the retinoblastoma gene product. Nature. 1991; 350(6314):160-2. DOI: 10.1038/350160a0. View