Protection of Aotus Monkeys from Malaria Infection by Immunization with Recombinant Hybrid Proteins

Overview

Journal Infect Immun

Publisher American Society for Microbiology

Specialty Allergy & Immunology

Date 1992 Jun 1

PMID 1587607

Citations 11

Authors

B Knapp

E Hundt

B Enders

H A Kupper

Affiliations

Soon will be listed here.

Abstract

On the basis of investigations of the malarial blood-stage antigens SERP, HRPII, and MSAI from Plasmodium falciparum, we chose two Escherichia coli-expressed hybrid proteins containing selected partial sequences of these antigens. Antibodies raised against both hybrid proteins in rabbits and Aotus monkeys recognize the corresponding P. falciparum polypeptides. In two independent trials with 13 animals, immunization of Aotus monkeys with either of the two hybrid proteins administered in a well-tolerated oil-based formulation protected the animals from an experimental P. falciparum infection.

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References

Knapp B, Nau U, Hundt E, Kupper H . A new blood stage antigen of Plasmodium falciparum highly homologous to the serine-stretch protein SERP. Mol Biochem Parasitol. 1991; 44(1):1-13. DOI: 10.1016/0166-6851(91)90215-r. View

Brent R, Ptashne M . Mechanism of action of the lexA gene product. Proc Natl Acad Sci U S A. 1981; 78(7):4204-8. PMC: 319757. DOI: 10.1073/pnas.78.7.4204. View

Roussilhon C, Hundt E, Agrapart M, Stuber W, Knapp B, Dubois P . Responses of T cells from sensitized donors to recombinant and synthetic peptides corresponding to sequences of the Plasmodium falciparum SERP antigen. Immunol Lett. 1990; 25(1-3):149-53. DOI: 10.1016/0165-2478(90)90107-2. View

Inselburg J, Bzik D, Li W, Green K, Kansopon J, Hahm B . Protective immunity induced in Aotus monkeys by recombinant SERA proteins of Plasmodium falciparum. Infect Immun. 1991; 59(4):1247-50. PMC: 257834. DOI: 10.1128/iai.59.4.1247-1250.1991. View

Siddiqui W, Tam L, Kramer K, Hui G, Case S, Yamaga K . Merozoite surface coat precursor protein completely protects Aotus monkeys against Plasmodium falciparum malaria. Proc Natl Acad Sci U S A. 1987; 84(9):3014-8. PMC: 304791. DOI: 10.1073/pnas.84.9.3014. View

Knapp B, Shaw A, Hundt E, Enders B, Kupper H . A histidin alanine rich recombinant antigen protects Aotus monkeys from P. falciparum infection. Behring Inst Mitt. 1988; (82):349-59. View

Bzik D, Li W, Horii T, Inselburg J . Amino acid sequence of the serine-repeat antigen (SERA) of Plasmodium falciparum determined from cloned cDNA. Mol Biochem Parasitol. 1988; 30(3):279-88. DOI: 10.1016/0166-6851(88)90097-7. View

Crisanti A, Muller H, Hilbich C, Sinigaglia F, Matile H, McKay M . Epitopes recognized by human T cells map within the conserved part of the GP190 of P. falciparum. Science. 1988; 240(4857):1324-6. DOI: 10.1126/science.2453924. View

Horton R, Hunt H, Ho S, Pullen J, Pease L . Engineering hybrid genes without the use of restriction enzymes: gene splicing by overlap extension. Gene. 1989; 77(1):61-8. DOI: 10.1016/0378-1119(89)90359-4. View

10.

Peterson M, Coppel R, McIntyre P, Langford C, Woodrow G, Brown G . Variation in the precursor to the major merozoite surface antigens of Plasmodium falciparum. Mol Biochem Parasitol. 1988; 27(2-3):291-301. DOI: 10.1016/0166-6851(88)90049-7. View

11.

Wellems T, Howard R . Homologous genes encode two distinct histidine-rich proteins in a cloned isolate of Plasmodium falciparum. Proc Natl Acad Sci U S A. 1986; 83(16):6065-9. PMC: 386439. DOI: 10.1073/pnas.83.16.6065. View

12.

Strebel K, Beck E, Strohmaier K, Schaller H . Characterization of foot-and-mouth disease virus gene products with antisera against bacterially synthesized fusion proteins. J Virol. 1986; 57(3):983-91. PMC: 252830. DOI: 10.1128/JVI.57.3.983-991.1986. View

13.

Holder A, Lockyer M, Odink K, Sandhu J, Nicholls S, Hillman Y . Primary structure of the precursor to the three major surface antigens of Plasmodium falciparum merozoites. Nature. 1985; 317(6034):270-3. DOI: 10.1038/317270a0. View

14.

Amann E, Ochs B, Abel K . Tightly regulated tac promoter vectors useful for the expression of unfused and fused proteins in Escherichia coli. Gene. 1988; 69(2):301-15. DOI: 10.1016/0378-1119(88)90440-4. View

15.

Holder A, Lockyer M, Hardy G . A hybrid gene to express protein epitopes from both sporozoite and merozoite surface antigens of Plasmodium falciparum. Parasitology. 1988; 97 ( Pt 3):373-82. DOI: 10.1017/s0031182000058790. View

16.

Patarroyo M, Amador R, Clavijo P, Moreno A, Guzman F, Romero P . A synthetic vaccine protects humans against challenge with asexual blood stages of Plasmodium falciparum malaria. Nature. 1988; 332(6160):158-61. DOI: 10.1038/332158a0. View

17.

Lyon J, Geller R, Haynes J, Chulay J, Weber J . Epitope map and processing scheme for the 195,000-dalton surface glycoprotein of Plasmodium falciparum merozoites deduced from cloned overlapping segments of the gene. Proc Natl Acad Sci U S A. 1986; 83(9):2989-93. PMC: 323432. DOI: 10.1073/pnas.83.9.2989. View

18.

Knapp B, Hundt E, Nau U, Kupper H . Molecular cloning, genomic structure and localization in a blood stage antigen of Plasmodium falciparum characterized by a serine stretch. Mol Biochem Parasitol. 1989; 32(1):73-83. DOI: 10.1016/0166-6851(89)90131-x. View

19.

Higgins D, McConnell D, Sharp P . Malarial proteinase?. Nature. 1989; 340(6235):604. DOI: 10.1038/340604a0. View

20.

Rock E, Marsh K, Saul A, Wellems T, Taylor D, Maloy W . Comparative analysis of the Plasmodium falciparum histidine-rich proteins HRP-I, HRP-II and HRP-III in malaria parasites of diverse origin. Parasitology. 1987; 95 ( Pt 2):209-27. DOI: 10.1017/s0031182000057681. View