Effects of Long-term Ethanol Consumption on Jejunal Lipase and Disaccharidase Activities in Male and Female Rats

Overview

Journal World J Gastroenterol

Publisher Baishideng Publishing Group

Specialty Gastroenterology

Date 2005 Apr 26

PMID 15849819

Citations 3

Authors

Chi-Chang Huang

Jiun-Rong Chen

Chieh-Chung Liu

Kung-tung Chen

Ming-Jer Shieh

Suh-Ching Yang

Affiliations

Soon will be listed here.

Abstract

Aim: To study the effect of long-term ethanol consumption on jejunal lipase and disaccharidase (sucrase, maltase, and lactase) activities in rats and its gender difference.

Methods: Age-matched male and female Wistar rats were fed control or ethanol-containing liquid diets for 12 wk following the Lieber-DeCarli model. According to both the plasma aspartate aminotransferase (AST) and alanine aminotransferase (ALT) activities, 40 rats were divided into four groups as follows: male control group (MC), male ethanol group (ME), female control group (FC), and female ethanol group (FE).

Results: After ethanol feeding for 12 wk, the results revealed that plasma AST and ALT activities of group ME were significantly increased by 58% and 92%, respectively, than those of group MC (P<0.05). Similarly, plasma AST and ALT activities of group FE were also significantly increased by 61% and 188%, respectively, than those of group FC (P<0.05). Fat accumulation was observed in both ethanol-treated groups, while fatty changes were more severe in group FE than those in group ME. The induction of hepatic microsomal cytochrome P450 2E1 (CYP2E1) was obviously seen in group ME and group FE, but was not detected in group MC and group FC. Jejunal lipase activity of group ME was significantly increased by 1.25-fold than that of group MC (P<0.05). In contrast to, sucrase, maltase, and lactase activities of group ME were significantly decreased by 63%, 62% and 67%, respectively, than those of group MC (P<0.05). Similarly, activities of these three enzymes of group FE were also significantly decreased by 43%, 46% and 52%, respectively, than those of group FC (P<0.05). There were no significant epithelial changes of the duodenal mucosa in any group.

Conclusion: Long-term ethanol consumption significantly can increase jejunal lipase and decrease jejunal disaccharidase activities in both male and female rats.

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References

Colantoni A, Idilman R, De Maria N, La Paglia N, Belmonte J, Wezeman F . Hepatic apoptosis and proliferation in male and female rats fed alcohol: role of cytokines. Alcohol Clin Exp Res. 2003; 27(7):1184-9. DOI: 10.1097/01.ALC.0000075834.52279.F9. View

Delgado M, Murillo M, Carreras O . Changes in the ileal disaccharidase activities in rats after long-term ethanol feeding. Alcohol Alcohol. 1996; 31(1):69-74. DOI: 10.1093/oxfordjournals.alcalc.a008118. View

Zucoloto S, Braulio V, Santos G, Ramalho F, Scandar M, DE FREITAS O . Effect of chronic ethanol consumption on the activities of residual small bowel brush-border enzymes after proximal jejunum resection in the rat. Alcohol Clin Exp Res. 1996; 20(1):152-5. DOI: 10.1111/j.1530-0277.1996.tb01058.x. View

Teschke R, Matsuzaki S, Ohnishi K, DECARLI L, Lieber C . Microsomal ethanol oxidizing system (MEOS): current status of its characterization and its role. Alcohol Clin Exp Res. 1977; 1(1):7-15. DOI: 10.1111/j.1530-0277.1977.tb05759.x. View

Salaspuro M, Lieber C . Metabolic consequences of chronic alcohol consumption: attenuation of hepatic redox changes despite enhanced capacity to eliminate ethanol. Curr Alcohol. 1979; 5:109-18. View

Lieber C, DECARLI L . Animal models of chronic ethanol toxicity. Methods Enzymol. 1994; 233:585-94. DOI: 10.1016/s0076-6879(94)33061-1. View

Becker U, Deis A, Sorensen T, Gronbaek M, Borch-Johnsen K, Muller C . Prediction of risk of liver disease by alcohol intake, sex, and age: a prospective population study. Hepatology. 1996; 23(5):1025-9. DOI: 10.1002/hep.510230513. View

Barona E, Pirola R, Leiber C . Small intestinal damage and changes in cell population produced by ethanol ingestion in the rat. Gastroenterology. 1974; 66(2):226-34. View

Pirola R, Lieber C . Energy wastage in rats given drugs that induce microsomal enzymes. J Nutr. 1975; 105(12):1544-8. DOI: 10.1093/jn/105.12.1544. View

10.

Nano J, Cefai D, Rampal P . Effects of ethanol on an intestinal epithelial cell line. Alcohol Clin Exp Res. 1990; 14(1):32-7. DOI: 10.1111/j.1530-0277.1990.tb00442.x. View

11.

Bode J, KNUPPEL H, Schwerk W, Bode C . Activities of cytoplasmic, mitochondrial and brush border enzymes in jejunal mucosa of chronic alcoholics. Z Gastroenterol. 1982; 20(4):228-33. View

12.

Koop D, Morgan E, Tarr G, Coon M . Purification and characterization of a unique isozyme of cytochrome P-450 from liver microsomes of ethanol-treated rabbits. J Biol Chem. 1982; 257(14):8472-80. View

13.

Tamai H, Horie Y, Kato S, Yokoyama H, Ishii H . Long-term ethanol feeding enhances susceptibility of the liver to orally administered lipopolysaccharides in rats. Alcohol Clin Exp Res. 2002; 26(8 Suppl):75S-80S. DOI: 10.1097/01.ALC.0000026981.32386.FD. View

14.

Pasqualini E, Nobili O, Verine A, Lombardo D . Effects of ethanol on the expression and secretion of bile salt-dependent lipase by pancreatic AR4-2J cells. Biochim Biophys Acta. 1998; 1408(1):44-54. DOI: 10.1016/s0925-4439(98)00054-4. View

15.

Nelson D, Kamataki T, Waxman D, Guengerich F, Estabrook R, Feyereisen R . The P450 superfamily: update on new sequences, gene mapping, accession numbers, early trivial names of enzymes, and nomenclature. DNA Cell Biol. 1993; 12(1):1-51. DOI: 10.1089/dna.1993.12.1. View

16.

McCarthy D, Nicholson J, Kim Y . Intestinal enzyme adaptation to normal diets of different composition. Am J Physiol. 1980; 239(6):G445-51. DOI: 10.1152/ajpgi.1980.239.6.G445. View

17.

Gavaler J . Sex-related differences in ethanol-induced liver disease: artifactual or real?. Alcohol Clin Exp Res. 1982; 6(2):186-96. DOI: 10.1111/j.1530-0277.1982.tb04961.x. View

18.

Dahlqvist A . Assay of intestinal disaccharidases. Anal Biochem. 1968; 22(1):99-107. DOI: 10.1016/0003-2697(68)90263-7. View

19.

Perlow W, BARAONA E, Lieber C . Symptomatic intestinal disaccharidase deficiency in alcoholics. Gastroenterology. 1977; 72(4 Pt 1):680-4. View

20.

Cunningham C, Spach P . The effect of chronic ethanol consumption on the lipids in liver mitochondria. Ann N Y Acad Sci. 1987; 492:181-92. DOI: 10.1111/j.1749-6632.1987.tb48667.x. View