Studies on Tsetse Midgut Factors That Induce Differentiation of Blood-stream Trypanosoma Brucei Brucei in Vitro

Overview

Journal Parasitol Res

Specialty Parasitology

Date 1992 Jan 1

PMID 1584740

Citations 10

Authors

M O Imbuga

E O Osir

V L Labongo

N Darji

L H Otieno

Affiliations

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Abstract

An in vitro system for studying the transformation of bloodstream forms of Trypanosoma brucei brucei into procylic (midgut) forms is described. In this system, transformation of the parasites was stimulated by Glossina morsitans morsitans midgut homogenates at 27 degrees C but not at 4 degrees C. The transformation-stimulating capacity was irreversibly destroyed by heating the midgut homogenates at 60 degrees C for 1 h. A correlation was established between the transformation activity of the midgut homogenates and trypsin activity. The protease inhibitors (soybean trypsin inhibitor and N-p-tosyl-L-lysine-chloromethyl-ketone) inhibited trypsin activity and completely blocked the transformation of the parasites. Furthermore, the midgut homogenates could induce transformation only in the presence of blood. These results provide evidence for the involvement of trypsin or trypsin-like enzymes within the tsetse midgut in stimulation of the transformation of bloodstream trypanosomes.

Citing Articles

Spiroplasma endosymbiont reduction of host lipid synthesis and Stomoxyn-like peptide contribute to trypanosome resistance in the tsetse fly Glossina fuscipes.

Awuoche E, Smallenberger G, Bruzzese D, Orfano A, Weiss B, Aksoy S PLoS Pathog. 2025; 21(1):e1012692.

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endosymbiont reduction of host lipid synthesis and Stomoxyn-like peptide contribute to trypanosome resistance in the tsetse fly .

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Molecular characterization of tsetse's proboscis and its response to Trypanosoma congolense infection.

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Independent pathways can transduce the life-cycle differentiation signal in Trypanosoma brucei.

Szoor B, Dyer N, Ruberto I, Acosta-Serrano A, Matthews K PLoS Pathog. 2013; 9(10):e1003689.

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Expression of procyclin mRNAs during cyclical transmission of Trypanosoma brucei.

Urwyler S, Vassella E, Van Den Abbeele J, Kunz Renggli C, Blundell P, Barry J PLoS Pathog. 2005; 1(3):e22.

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References

Yeates R, Steiger S . Ultrastructural damage of in vitro cultured ookinetes of Plasmodium gallinaceum (Brumpt) by purified proteinases of susceptible Aedes aegypti (L.). Z Parasitenkd. 1981; 66(1):93-7. DOI: 10.1007/BF00941949. View

Kaminsky R, Beaudoin E, Cunningham I . Cultivation of the life cycle stages of Trypanosoma brucei sspp. Acta Trop. 1988; 45(1):33-43. View

Isola E, Lammel E, Gonzalez Cappa S . Trypanosoma cruzi: differentiation after interaction of epimastigotes and Triatoma infestans intestinal homogenate. Exp Parasitol. 1986; 62(3):329-35. DOI: 10.1016/0014-4894(86)90039-1. View

Bienen E, Hammadi E, Hill G . Initiation of trypanosome transformation from bloodstream trypomastigotes to procyclic trypomastigotes. J Parasitol. 1980; 66(4):680-2. View

Bienen E, Hill G, Shin K . Elaboration of mitochondrial function during Trypanosoma brucei differentiation. Mol Biochem Parasitol. 1983; 7(1):75-86. DOI: 10.1016/0166-6851(83)90118-4. View

Otieno L, Darji N, Onyango P, Mpanga E . Some observations on factors associated with the development of Trypanosoma brucei brucei infections in Glossina morsitans morsitans. Acta Trop. 1983; 40(2):113-20. View

Czichos J, Nonnengaesser C, Overath P . Trypanosoma brucei: cis-aconitate and temperature reduction as triggers of synchronous transformation of bloodstream to procyclic trypomastigotes in vitro. Exp Parasitol. 1986; 62(2):283-91. DOI: 10.1016/0014-4894(86)90033-0. View

Grab D, Bwayo J . Isopycnic isolation of African trypanosomes on Percoll gradients formed in situ. Acta Trop. 1982; 39(4):363-6. View

Gooding R, Rolseth B . Digestive processes of haematophagous insects. XI. Partial purification and some properties of six proteolytic enzymes from the tsetse fly Glossina morsitans morsitans Westwood (Diptera: Glossinidae). Can J Zool. 1976; 54(11):1950-9. DOI: 10.1139/z76-226. View

10.

Brun R, Schonenberger M . Stimulating effect of citrate and cis-Aconitate on the transformation of Trypanosoma brucei bloodstream forms to procyclic forms in vitro. Z Parasitenkd. 1981; 66(1):17-24. DOI: 10.1007/BF00941941. View

11.

Englund P, Hajduk S, Marini J . The molecular biology of trypanosomes. Annu Rev Biochem. 1982; 51:695-726. DOI: 10.1146/annurev.bi.51.070182.003403. View

12.

Yabu Y, Takayanagi T . Trypsin-stimulated transformation of Trypanosoma brucei gambiense bloodstream forms to procyclic forms in vitro. Parasitol Res. 1988; 74(6):501-6. DOI: 10.1007/BF00531625. View

13.

Frevert U, Herzberg F, Reinwald E, RISSE H . Morphological changes in Trypanosoma congolense after proteolytic removal of the surface coat. J Ultrastruct Mol Struct Res. 1986; 94(2):140-8. DOI: 10.1016/0889-1605(86)90060-1. View

14.

Overath P, Czichos J, Stock U, Nonnengaesser C . Repression of glycoprotein synthesis and release of surface coat during transformation of Trypanosoma brucei. EMBO J. 1983; 2(10):1721-8. PMC: 555349. DOI: 10.1002/j.1460-2075.1983.tb01648.x. View

15.

Roditi I, Pearson T . The procyclin coat of African trypanosomes (or the not-so-naked trypanosome). Parasitol Today. 1990; 6(3):79-82. DOI: 10.1016/0169-4758(90)90216-q. View

16.

Bienen E, Hammadi E, Hill G . Trypanosoma brucei: biochemical and morphological changes during in vitro transformation of bloodstream- to procyclic-trypomastigotes. Exp Parasitol. 1981; 51(3):408-17. DOI: 10.1016/0014-4894(81)90128-4. View

17.

Durieux P, Schutz P, Brun R, Kohler P . Alterations in Krebs cycle enzyme activities and carbohydrate catabolism in two strains of Trypanosoma brucei during in vitro differentiation of their bloodstream to procyclic stages. Mol Biochem Parasitol. 1991; 45(1):19-27. DOI: 10.1016/0166-6851(91)90023-y. View

18.

GHIOTTO V, Brun R, Jenni L, Hecker H . Trypanosoma brucei: morphometric changes and loss of infectivity during transformation of bloodstream forms to procyclic culture forms in vitro. Exp Parasitol. 1979; 48(3):447-56. DOI: 10.1016/0014-4894(79)90129-2. View

19.

Vickerman K . Polymorphism and mitochondrial activity in sleeping sickness trypanosomes. Nature. 1965; 208(5012):762-6. DOI: 10.1038/208762a0. View

20.

Erlanger B, KOKOWSKY N, Cohen W . The preparation and properties of two new chromogenic substrates of trypsin. Arch Biochem Biophys. 1961; 95:271-8. DOI: 10.1016/0003-9861(61)90145-x. View