Escherichia Coli K1 RS218 Interacts with Human Brain Microvascular Endothelial Cells Via Type 1 Fimbria Bacteria in the Fimbriated State

Overview

Journal Infect Immun

Publisher American Society for Microbiology

Specialty Allergy & Immunology

Date 2005 Apr 23

PMID 15845498

Citations 60

Authors

Ching-Hao Teng

Mian Cai

Sooan Shin

Yi Xie

Kee-Jun Kim

Naveed Ahmed Khan

Francescopaolo Di Cello

Kwang Sik Kim

Affiliations

Soon will be listed here.

Abstract

Escherichia coli K1 is a major gram-negative organism causing neonatal meningitis. E. coli K1 binding to and invasion of human brain microvascular endothelial cells (HBMEC) are a prerequisite for E. coli penetration into the central nervous system in vivo. In the present study, we showed using DNA microarray analysis that E. coli K1 associated with HBMEC expressed significantly higher levels of the fim genes compared to nonassociated bacteria. We also showed that E. coli K1 binding to and invasion of HBMEC were significantly decreased with its fimH deletion mutant and type 1 fimbria locked-off mutant, while they were significantly increased with its type 1 fimbria locked-on mutant. E. coli K1 strains associated with HBMEC were predominantly type 1 fimbria phase-on (i.e., fimbriated) bacteria. Taken together, we showed for the first time that type 1 fimbriae play an important role in E. coli K1 binding to and invasion of HBMEC and that type 1 fimbria phase-on E. coli is the major population interacting with HBMEC.

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