Overexpression of Anti-apoptotic Mcl-1 in Testicular Germ Cell Tumours

Overview

Journal Histopathology

Specialties Anatomy & Histology
Pathology

Date 2005 Apr 22

PMID 15842635

Citations 5

Authors

M Sano

Y Nakanishi

H Yagasaki

T Honma

T Oinuma

Y Obana

A Suzuki

N Nemoto

Affiliations

Soon will be listed here.

Abstract

Aims: To determine the expression of Mcl-1 in testicular germ cell tumours in order to clarify the role of this anti-apoptotic factor in these tumours. Various members of the Bcl-2 family have been implicated in the apoptotic mechanisms regulating germ cell apoptosis. Mcl-1 is an anti-apoptotic Bcl-2 family member and has recently been reported to be related to the progression of malignancy; however, the involvement of Mcl-1 in the development of germ cell tumours is still unknown.

Methods And Results: Mcl-1 expression in testicular germ cell tumours was investigated by immunohistochemistry and reverse transcriptase-polymerase chain reaction (RT-PCR). By immunohistochemistry, overexpression of Mcl-1 was present in all germ cell tumours that were studied, including embryonal carcinoma and yolk sac tumour, as well as choriocarcinoma and teratoma. In teratomas, Mcl-1 was widely distributed in the epithelial, myogenic, neural and mesenchymal components. RT-PCR analysis after microdissection revealed high levels of Mcl-1 mRNA in all tumour variants compared with non-neoplastic germ cells.

Conclusion: Overexpression of anti-apoptotic Mcl-1 may function to enhance the viability of testicular germ cells, thereby leading to tumorigenesis.

Citing Articles

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Wu X, Luo Q, Liu Z Cell Death Dis. 2020; 11(7):556.

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Cisplatin Resistance in Testicular Germ Cell Tumors: Current Challenges from Various Perspectives.

Lobo J, Jeronimo C, Henrique R Cancers (Basel). 2020; 12(6).

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Thr 163 phosphorylation causes Mcl-1 stabilization when degradation is independent of the adjacent GSK3-targeted phosphodegron, promoting drug resistance in cancer.

Nifoussi S, Vrana J, Domina A, De Biasio A, Gui J, Gregory M PLoS One. 2012; 7(10):e47060.

PMID: 23056582 PMC: 3467206. DOI: 10.1371/journal.pone.0047060.

Male germ cell apoptosis: regulation and biology.

Shaha C, Tripathi R, Mishra D Philos Trans R Soc Lond B Biol Sci. 2010; 365(1546):1501-15.

PMID: 20403866 PMC: 2871916. DOI: 10.1098/rstb.2009.0124.

Mcl-1, an anti-apoptotic Bcl-2 family member, essentially overlaps with insulin-producing cells in neonatal nesidioblastosis.

Sano M, Hayashi E, Murakami H, Kishimoto H, Fukuzawa R, Nemoto N Virchows Arch. 2008; 452(4):469-70.

PMID: 18236072 DOI: 10.1007/s00428-007-0567-4.