Bacillus Subtilis Paraquat Resistance is Directed by SigmaM, an Extracytoplasmic Function Sigma Factor, and is Conferred by YqjL and BcrC

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 2005 Apr 20

PMID 15838020

Citations 25

Authors

Min Cao

Charles M Moore

John D Helmann

Affiliations

Soon will be listed here.

Abstract

A Bacillus subtilis sigM null mutant, lacking the extracytoplasmic function sigma(M) protein, was sensitive to paraquat (PQ), a superoxide-generating reagent, but not to the redox stress-inducing compounds hydrogen peroxide, cumene hydroperoxide, t-butyl hydroperoxide, or diamide. Surprisingly, a sigM mutant was only sensitive to superoxide-generating compounds with a dipyridyl ring such as PQ, ethyl viologen, benzyl viologen, and diquat but not to menadione, plumbagin, pyrogallol, or nitrofurantoin. Mutational analysis of candidate sigma(M)-regulated genes revealed that both YqjL, a putative hydrolase, and BcrC, a bacitracin resistance protein, were involved in PQ resistance. Expression of yqjL, but not bcrC, from a xylose-inducible promoter restored PQ resistance to the sigM mutant.

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