Specific NF-kappa B Subunits Act in Concert with Tat to Stimulate Human Immunodeficiency Virus Type 1 Transcription

Overview

Journal J Virol

Publisher American Society for Microbiology

Date 1992 Jun 1

PMID 1583734

Citations 42

Authors

J Liu

N D Perkins

R M Schmid

G J Nabel

Affiliations

Soon will be listed here.

Abstract

NF-kappa B is a protein complex which functions in concert with the tat-I gene product to stimulate human immunodeficiency virus (HIV) transcription. To determine whether specific members of the NF-kappa B family contribute to this effect, we have examined the abilities of different NF-kappa B subunits to act with Tat-I to stimulate transcription of HIV in Jurkat T-leukemia cells. We have found that the p49(100) DNA binding subunit, together with p65, can act in concert with Tat-I to stimulate the expression of HIV-CAT plasmid. Little effect was observed with 50-kDa forms of p105 NF-kappa B or rel, in combination with p65 or full-length c-rel, which do not stimulate the HIV enhancer in these cells. These findings suggest that the combination of p49(100) and p65 NF-kappa B can act in concert with the tat-I gene product to stimulate the synthesis of HIV RNA.

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