Helicobacter Pylori DnaB Helicase Can Bypass Escherichia Coli DnaC Function in Vivo

Overview

Journal Biochem J

Specialty Biochemistry

Date 2005 Apr 20

PMID 15836434

Citations 15

Authors

Rajesh K Soni

Parul Mehra

Gauranga Mukhopadhyay

Suman Kumar Dhar

Affiliations

Soon will be listed here.

Abstract

In Escherichia coli, DnaC is essential for loading DnaB helicase at oriC (the origin of chromosomal DNA replication). The question arises as to whether this model can be generalized to other species, since many eubacterial species fail to possess dnaC in their genomes. Previously, we have reported the characterization of HpDnaB (Helicobacter pylori DnaB) both in vitro and in vivo. Interestingly, H. pylori does not have a DnaC homologue. Using two different E. coli dnaC (EcdnaC) temperature-sensitive mutant strains, we report here the complementation of EcDnaC function by HpDnaB in vivo. These observations strongly suggest that HpDnaB can bypass EcDnaC activity in vivo.

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