Changes in the Capillarity of Skeletal Muscle in the Growing Rat

Overview

Journal Pflugers Arch

Specialty Physiology

Date 1979 Jun 12

PMID 158170

Citations 34

Authors

E Ripoll

A H Sillau

N Banchero

Affiliations

Soon will be listed here.

Abstract

Capillary density (CD), capillary to fiber ratio (C/F), fiber cross sectional area (FCSA) and fiber composition were measured in the soleus and the gastrocnemius (medial head) muscles of rats weighing between 99 and 666 g. Muscle samples obtained from the anesthetized animal were rapidly frozen (-130 degrees C) sliced transversely at 16--18 micrometers, and treated histochemically by the ATPase method after preincubation at pH's of 4.0 and 4.4 to visualize capillaries and typify fibers. In both muscles the FCSA was positively related to body weight (BW) and muscle weight. At a given BW, the FCSA of the soleus was greater than that of the gastrocnemius. In both muscles CD decreased hyperbolically with FCSA (soleus: CD = 1.0613 X 10(6)/FCSA + 298.71; gastrocnemium: CD = 1.0349 X 10(6)/FCSA + 240.74). At the same time a positive linear correlation between C/F and FCSA was found (soleus: C/F = 3.92 X 10(-4) FCSA + 0.82; gastrocnemius: C/F = 2.90 X 10(-4) FCSA + 0.93). At a given FCSA, CD and C/F were greater in the soleus than in the gastrocnemius because of differences in fiber composition between the two muscles. The soleus had only oxidative fibers (STO and FTOG) whereas the gastrocnemius had 54% glycolytic fibers (FTG). The very large variability in CD and C/F values reported in the literature could, in part, be due to the differences in capillarity observed with maturation. A change in fiber composition with BW was observed in the soleus, but no systematic change occurred in the gastrocnemius.

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References

Hermansen L, Wachtlova M . Capillary density of skeletal muscle in well-trained and untrained men. J Appl Physiol. 1971; 30(6):860-3. DOI: 10.1152/jappl.1971.30.6.860. View

Myrhage R . Capillary supply of the muscle fibre population in hindlimb muscles of the cat. Acta Physiol Scand. 1978; 103(1):19-30. DOI: 10.1111/j.1748-1716.1978.tb06186.x. View

PADYKULA H, Herman E . The specificity of the histochemical method for adenosine triphosphatase. J Histochem Cytochem. 1955; 3(3):170-95. DOI: 10.1177/3.3.170. View

Henquell L, Odoroff C, HONIG C . Coronary intercapillary distance during growth: relation to PtO2 and aerobic capacity. Am J Physiol. 1976; 231(6):1852-9. DOI: 10.1152/ajplegacy.1976.231.6.1852. View

Sillau A, Banchero N . Visualization of capillaries in skeletal muscle by the ATPase reaction. Pflugers Arch. 1977; 369(3):269-71. DOI: 10.1007/BF00582194. View

GAUTHIER G, LOWEY S, Hobbs A . Fast and slow myosin in developing muscle fibres. Nature. 1978; 274(5666):25-9. DOI: 10.1038/274025a0. View

Carrow R, Brown R, VAN HUSS W . Fiber sizes and capillary to fiber ratios in skeletal muscle of exercised rats. Anat Rec. 1967; 159(1):33-9. DOI: 10.1002/ar.1091590106. View

Peter J, Barnard R, Edgerton V, Gillespie C, Stempel K . Metabolic profiles of three fiber types of skeletal muscle in guinea pigs and rabbits. Biochemistry. 1972; 11(14):2627-33. DOI: 10.1021/bi00764a013. View

Brodal P, Ingjer F, Hermansen L . Capillary supply of skeletal muscle fibers in untrained and endurance-trained men. Am J Physiol. 1977; 232(6):H705-12. DOI: 10.1152/ajpheart.1977.232.6.H705. View

10.

Ingjer F, Brodal P . Capillary supply of skeletal muscle fibers in untrained and endurance-trained women. Eur J Appl Physiol Occup Physiol. 1978; 38(4):291-9. DOI: 10.1007/BF00423112. View

11.

ROMANUL F . CAPILLARY SUPPLY AND METABOLISM OF MUSCLE FIBERS. Arch Neurol. 1965; 12:497-509. DOI: 10.1001/archneur.1965.00460290053007. View

12.

Mai J, Edgerton V, Barnard R . Capillarity of red, white and intermediate muscle fibers in trained and untrained guinea pigs. Experientia. 1970; 26(11):1222-3. DOI: 10.1007/BF01897977. View

13.

Wittenberg J . Myoglobin-facilitated oxygen diffusion: role of myoglobin in oxygen entry into muscle. Physiol Rev. 1970; 50(4):559-636. DOI: 10.1152/physrev.1970.50.4.559. View

14.

Sillau A, Banchero N . Skeletal muscle fiber size and capillarity. Proc Soc Exp Biol Med. 1978; 158(2):288-91. DOI: 10.3181/00379727-158-4990. View

15.

Cassin S, Gilbert R, Bunnell C, Johnson E . Capillary development during exposure to chronic hypoxia. Am J Physiol. 1971; 220(2):448-51. DOI: 10.1152/ajplegacy.1971.220.2.448. View

16.

Sillau A, Banchero N . Effect of maturation on capillary density, fiber size and composition in rat skeletal muscle. Proc Soc Exp Biol Med. 1977; 154(3):461-6. DOI: 10.3181/00379727-154-39694. View

17.

Faulkner J, Maxwell L, Brook D, Lieberman D . Adaptation of guinea pig plantaris muscle fibers to endurance training. Am J Physiol. 1971; 221(1):291-7. DOI: 10.1152/ajplegacy.1971.221.1.291. View

18.

Kugelberg E . Adaptive transformation of rat soleus motor units during growth. J Neurol Sci. 1976; 27(3):269-89. DOI: 10.1016/0022-510x(76)90001-0. View