Mature DIABLO/Smac is Produced by the IMP Protease Complex on the Mitochondrial Inner Membrane

Overview

Journal Mol Biol Cell

Specialties Cell Biology
Molecular Biology

Date 2005 Apr 9

PMID 15814844

Citations 37

Authors

Lena Burri

Yvan Strahm

Christine J Hawkins

Ian E Gentle

Michelle A Puryer

Anne Verhagen

Bernard Callus

David Vaux

Trevor Lithgow

Affiliations

Soon will be listed here.

Abstract

DIABLO/Smac is a mitochondrial protein that can promote apoptosis by promoting the release and activation of caspases. To do so, DIABLO/Smac must first be processed by a mitochondrial protease and then released into the cytosol, and we show this in an intact cellular system. We propose that the precursor form of DIABLO/Smac enters the mitochondria through a stop-transfer pathway and is processed to its active form by the inner membrane peptidase (IMP) complex. Catalytic subunits of the mammalian IMP complex were identified based on sequence conservation and functional complementation, and the novel sequence motif RX(5)P in Imp1 and NX(5)S in Imp2 distinguish the two catalytic subunits. DIABLO/Smac is one of only a few specific proteins identified as substrates for the IMP complex in the mitochondrial intermembrane space.

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