Association of 2'-5' Oligoribonucleotides

Overview

Journal Nucleic Acids Res

Publisher Oxford University Press

Specialty Biochemistry

Date 1992 Apr 11

PMID 1579460

Citations 15

Authors

R Kierzek

L He

D H Turner

Affiliations

Soon will be listed here.

Abstract

Oligoribonucleotides with 2'-5' linkages have been synthesized on solid support. UV melting and CD experiments indicate complementary strands associate to give complexes with melting temperatures 30 to 40 degrees C lower than for duplexes formed by 3'-5' oligoribonucleotides with the same sequence. UV melting and imino proton NMR spectra and NOEs for (2'-5') CGGCGCCG are consistent with formation of an antiparallel duplex. The results suggest greater duplex stability was one factor favoring 3'-5' over 2'-5' linkages in evolution.

Citing Articles

Hybrid DNA/RNA nanostructures with 2'-5' linkages.

Chandrasekaran A, Mathivanan J, Ebrahimi P, Vilcapoma J, Chen A, Halvorsen K Nanoscale. 2020; 12(42):21583-21590.

PMID: 33089274 PMC: 7644649. DOI: 10.1039/d0nr05846g.

2'/3' Regioselectivity of Enzyme-Free Copying of RNA Detected by NMR.

Motsch S, Pfeffer D, Richert C Chembiochem. 2020; 21(14):2013-2018.

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Structural insights into RNA duplexes with multiple 2΄-5΄-linkages.

Shen F, Luo Z, Liu H, Wang R, Zhang S, Gan J Nucleic Acids Res. 2016; 45(6):3537-3546.

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Structural insights into the effects of 2'-5' linkages on the RNA duplex.

Sheng J, Li L, Engelhart A, Gan J, Wang J, Szostak J Proc Natl Acad Sci U S A. 2014; 111(8):3050-5.

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Prebiotically plausible oligoribonucleotide ligation facilitated by chemoselective acetylation.

Bowler F, Chan C, Duffy C, Gerland B, Islam S, Powner M Nat Chem. 2013; 5(5):383-9.

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