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Fluctuation Correlation Spectroscopy with a Laser-scanning Microscope: Exploiting the Hidden Time Structure

Overview
Journal Biophys J
Publisher Cell Press
Specialty Biophysics
Date 2005 Mar 29
PMID 15792971
Citations 88
Authors
Michelle A Digman
Parijat Sengupta
Paul W Wiseman
Claire M Brown
Alan R Horwitz
Enrico Gratton
Affiliations
Soon will be listed here.
Abstract

Images obtained with a laser-scanning microscope contain a time structure that can be exploited to measure fast dynamics of molecules in solution and in cells. The spatial correlation approach provides a simple algorithm to extract this information. We describe the analysis used to process laser-scanning images of solutions and cells to obtain molecular diffusion constant in the microsecond to second timescale.

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