Genetic Diversity of Multidrug-resistant Mycobacterium Tuberculosis Isolates and Identification of 11 Novel RpoB Alleles in Taiwan

Overview

Journal J Clin Microbiol

Specialty Microbiology

Date 2005 Mar 8

PMID 15750115

Citations 19

Authors

Ruwen Jou

Huang-Yau Chen

Chen-Yuan Chiang

Ming-Chih Yu

Ih-Jen Su

Affiliations

Soon will be listed here.

Abstract

Of 162 multidrug-resistant Mycobacterium tuberculosis isolates from Taiwan, 60.5% were found to belong to the Beijing family on the basis of spoligotyping results. IS6110 restriction fragment length polymorphism fingerprinting showed genetic diversity among the multidrug-resistant isolates. Furthermore, 90.1% of the multidrug-resistant isolates had mutations in the rpoB gene, and 11 novel alleles were recognized.

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References

Yu M, Suo J, Chiang C, Bai K, Lin T, Luh K . Initial drug resistance of Mycobacterium tuberculosis in Taiwan. J Formos Med Assoc. 1997; 96(11):890-4. View

Moss A, Alland D, Telzak E, Hewlett Jr D, Sharp V, Chiliade P . A city-wide outbreak of a multiple-drug-resistant strain of Mycobacterium tuberculosis in New York. Int J Tuberc Lung Dis. 1997; 1(2):115-21. View

Pozzi G, Meloni M, Iona E, Orru G, Thoresen O, Ricci M . rpoB mutations in multidrug-resistant strains of Mycobacterium tuberculosis isolated in Italy. J Clin Microbiol. 1999; 37(4):1197-9. PMC: 88675. DOI: 10.1128/JCM.37.4.1197-1199.1999. View

Tribuddharat C, Fennewald M . Integron-mediated rifampin resistance in Pseudomonas aeruginosa. Antimicrob Agents Chemother. 1999; 43(4):960-2. PMC: 89236. DOI: 10.1128/AAC.43.4.960. View

Hirano K, Abe C, Takahashi M . Mutations in the rpoB gene of rifampin-resistant Mycobacterium tuberculosis strains isolated mostly in Asian countries and their rapid detection by line probe assay. J Clin Microbiol. 1999; 37(8):2663-6. PMC: 85308. DOI: 10.1128/JCM.37.8.2663-2666.1999. View

Yuen L, Leslie D, Coloe P . Bacteriological and molecular analysis of rifampin-resistant Mycobacterium tuberculosis strains isolated in Australia. J Clin Microbiol. 1999; 37(12):3844-50. PMC: 85826. DOI: 10.1128/JCM.37.12.3844-3850.1999. View

Valim A, Rossetti M, Ribeiro M, Zaha A . Mutations in the rpoB gene of multidrug-resistant Mycobacterium tuberculosis isolates from Brazil. J Clin Microbiol. 2000; 38(8):3119-22. PMC: 87207. DOI: 10.1128/JCM.38.8.3119-3122.2000. View

Espinal M, Laszlo A, Simonsen L, Boulahbal F, Kim S, Reniero A . Global trends in resistance to antituberculosis drugs. World Health Organization-International Union against Tuberculosis and Lung Disease Working Group on Anti-Tuberculosis Drug Resistance Surveillance. N Engl J Med. 2001; 344(17):1294-303. DOI: 10.1056/NEJM200104263441706. View

Garcia L, Rebollo M, Tercero J, Chaves F . Mutations in the rpoB gene of rifampin-resistant Mycobacterium tuberculosis isolates in Spain and their rapid detection by PCR-enzyme-linked immunosorbent assay. J Clin Microbiol. 2001; 39(5):1813-8. PMC: 88031. DOI: 10.1128/JCM.39.5.1813-1818.2001. View

10.

Mani C, Selvakumar N, Narayanan S, Narayanan P . Mutations in the rpoB gene of multidrug-resistant Mycobacterium tuberculosis clinical isolates from India. J Clin Microbiol. 2001; 39(8):2987-90. PMC: 88277. DOI: 10.1128/JCM.39.8.2987-2990.2001. View

11.

Bartfai Z, Somoskovi A, Kodmon C, Szabo N, Puskas E, Kosztolanyi L . Molecular characterization of rifampin-resistant isolates of Mycobacterium tuberculosis from Hungary by DNA sequencing and the line probe assay. J Clin Microbiol. 2001; 39(10):3736-9. PMC: 88421. DOI: 10.1128/JCM.39.10.3736-3739.2001. View

12.

Qian L, Abe C, Lin T, Yu M, Cho S, Wang S . rpoB genotypes of Mycobacterium tuberculosis Beijing family isolates from East Asian countries. J Clin Microbiol. 2002; 40(3):1091-4. PMC: 120282. DOI: 10.1128/JCM.40.3.1091-1094.2002. View

13.

Glynn J, Whiteley J, Bifani P, Kremer K, van Soolingen D . Worldwide occurrence of Beijing/W strains of Mycobacterium tuberculosis: a systematic review. Emerg Infect Dis. 2002; 8(8):843-9. PMC: 2732522. DOI: 10.3201/eid0805.020002. View

14.

Tracevska T, Jansone I, Broka L, Marga O, Baumanis V . Mutations in the rpoB and katG genes leading to drug resistance in Mycobacterium tuberculosis in Latvia. J Clin Microbiol. 2002; 40(10):3789-92. PMC: 130873. DOI: 10.1128/JCM.40.10.3789-3792.2002. View

15.

Cavusoglu C, Hilmioglu S, Guneri S, Bilgic A . Characterization of rpoB mutations in rifampin-resistant clinical isolates of Mycobacterium tuberculosis from Turkey by DNA sequencing and line probe assay. J Clin Microbiol. 2002; 40(12):4435-8. PMC: 154651. DOI: 10.1128/JCM.40.12.4435-4438.2002. View

16.

Hwang H, Chang C, Chang L, Chang S, Chang Y, Chen Y . Characterization of rifampicin-resistant Mycobacterium tuberculosis in Taiwan. J Med Microbiol. 2003; 52(Pt 3):239-245. DOI: 10.1099/jmm.0.05045-0. View

17.

Yue J, Shi W, Xie J, Li Y, Zeng E, Wang H . Mutations in the rpoB gene of multidrug-resistant Mycobacterium tuberculosis isolates from China. J Clin Microbiol. 2003; 41(5):2209-12. PMC: 154692. DOI: 10.1128/JCM.41.5.2209-2212.2003. View

18.

Alexander D, Jones J, Liu J . A rifampin-hypersensitive mutant reveals differences between strains of Mycobacterium smegmatis and presence of a novel transposon, IS1623. Antimicrob Agents Chemother. 2003; 47(10):3208-13. PMC: 201156. DOI: 10.1128/AAC.47.10.3208-3213.2003. View

19.

Liaw Y, Hsueh P, Yu C, Wang S, Yang P, Luh K . Drug resistance pattern of Mycobacterium tuberculosis in a university hospital in Taiwan, 1998-2002. J Formos Med Assoc. 2004; 103(9):671-7. View

20.

Ovchinnikov YuA , Monastyrskaya G, Gubanov V, Lipkin V, Sverdlov E, Kiver I . Primary structure of Escherichia coli RNA polymerase nucleotide substitution in the beta subunit gene of the rifampicin resistant rpoB255 mutant. Mol Gen Genet. 1981; 184(3):536-8. DOI: 10.1007/BF00352535. View