Epidemiology of Human Sporotrichosis Investigated by Amplified Fragment Length Polymorphism

Overview

Journal J Clin Microbiol

Specialty Microbiology

Date 2005 Mar 8

PMID 15750106

Citations 15

Authors

Edgar Neyra

Pierre-Alain Fonteyne

Danielle Swinne

Frederic Fauche

Beatriz Bustamante

Nicole Nolard

Affiliations

Soon will be listed here.

Abstract

Amplified fragment length polymorphism (AFLP) was used to analyze the genetic diversity of Peruvian strains of Sporothrix schenckii and to compare them to a panel of non-Peruvian strains. AFLP analysis suggests that the Peruvian strains can be divided into two homogeneous clusters with no reference to geographical origin or the clinical form of sporotrichosis. The strains from abroad present heterogeneous profiles, with the Bolivian strain and the Colombian strains related to one of the Peruvian population. Sequencing of internal transcribed spacer 2, used to examine the relationships over a longer distance, confirmed the division of Peruvian strains into two populations that can be identified on the basis of a single but specific sequence divergence. This paper introduces automated AFLP analysis as a valuable tool for further investigation of the epidemiology and ecology of S. schenckii.

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