» Articles » PMID: 15747251

Use of RNA Interference to Prevent Lethal Murine West Nile Virus Infection

Overview
Journal J Infect Dis
Date 2005 Mar 5
PMID 15747251
Citations 47
Authors
Affiliations
Soon will be listed here.
Abstract

West Nile virus causes fatal encephalitis in humans, mice, and other vertebrates. In the present study, we demonstrate that small interfering RNAs (siRNAs) inhibit West Nile virus replication in vitro. Moreover, the administration of siRNAs to mice by hydrodynamic injection 24 h before challenge with an intraperitoneal inoculum of West Nile virus reduced the viral load and afforded partial protection from lethal infection. These data show the efficacy of the prophylactic use of siRNAs against a viral infection in vivo and suggest new strategies to combat West Nile virus.

Citing Articles

Identification of Benzothiophene-Derived Inhibitors of Flaviviruses by Targeting RNA-Dependent RNA Polymerase.

Wang L, Karim S, Hand A, Brunkhorst R, Petersen M, Altman S Viruses. 2025; 17(2).

PMID: 40006900 PMC: 11861172. DOI: 10.3390/v17020145.


A novel nanocomposite drug delivery system for SARS-CoV-2 infections.

Chintapula U, Karim S, Iyer P, Asokan-Sheeja H, Neupane B, Nazneen F Nanoscale Adv. 2024; 6(15):3747-3758.

PMID: 39050946 PMC: 11265598. DOI: 10.1039/d4na00361f.


An effective live-attenuated Zika vaccine candidate with a modified 5' untranslated region.

Nazneen F, Thompson E, Blackwell C, Bai J, Huang F, Bai F NPJ Vaccines. 2023; 8(1):50.

PMID: 37005424 PMC: 10066991. DOI: 10.1038/s41541-023-00650-w.


Quantification of West Nile Virus by Plaque-Forming Assay.

Neupane B, Bai F Methods Mol Biol. 2022; 2585:9-14.

PMID: 36331760 PMC: 10719966. DOI: 10.1007/978-1-0716-2760-0_2.


Mouse Trophoblast Cells Can Provide IFN-Based Antiviral Protection to Embryonic Stem Cells via Paracrine Signaling.

Fendereski M, Neupane B, Nazneen F, Bai F, Guo Y J Immunol. 2022; 208(12):2761-2770.

PMID: 35649628 PMC: 9308691. DOI: 10.4049/jimmunol.2100679.