Quantitative Molecular Assay for Fingerprinting Microbial Communities of Wastewater and Estrogen-degrading Consortia

Overview

Journal Appl Environ Microbiol

Specialties Environmental Health
Microbiology

Date 2005 Mar 5

PMID 15746346

Citations 13

Authors

Chang-Ping Yu

Rajiv Ahuja

Gary Sayler

Kung-Hui Chu

Affiliations

Soon will be listed here.

Abstract

A quantitative fingerprinting method, called the real-time terminal restriction fragment length polymorphism (real-time-t-RFLP) assay, was developed for simultaneous determination of microbial diversity and abundance within a complex community. The real-time-t-RFLP assay was developed by incorporating the quantitative feature of real-time PCR and the fingerprinting feature of t-RFLP analysis. The assay was validated by using a model microbial community containing three pure strains, an Escherichia coli strain (gram negative), a Pseudomonas fluorescens strain (gram negative), and a Bacillus thuringiensis strain (gram positive). Subsequently, the real-time-t-RFLP assay was applied to and proven to be useful for environmental samples; the richness and abundance of species in microbial communities (expressed as the number of 16S rRNA gene copies of each ribotype per milliliter) of wastewater and estrogen-degrading consortia (enriched with 17alpha-estradiol, 17beta-estradiol, or estrone) were successfully characterized. The results of this study strongly suggested that the real-time-t-RFLP assay can be a powerful molecular tool for gaining insight into microbial communities in various engineered systems and natural habitats.

Citing Articles

BODIPY-Labeled Estrogens for Fluorescence Analysis of Environmental Microbial Degradation.

Felion C, Lopez-Gonzalez R, Sewell A, Marquez R, Gauchotte-Lindsay C ACS Omega. 2022; 7(45):41284-41295.

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Acclimation of Culturable Bacterial Communities under the Stresses of Different Organic Compounds.

Wang H, Zhang S, Pratush A, Ye X, Xie J, Wei H Front Microbiol. 2018; 9:225.

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Estrogen receptor beta as target for colorectal cancer prevention.

Williams C, DiLeo A, Niv Y, Gustafsson J Cancer Lett. 2015; 372(1):48-56.

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Diet complexity and estrogen receptor β status affect the composition of the murine intestinal microbiota.

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High-sensitivity stable-isotope probing by a quantitative terminal restriction fragment length polymorphism protocol.

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