Synthetic Lethality of Retinoblastoma Mutant Cells in the Drosophila Eye by Mutation of a Novel Peptidyl Prolyl Isomerase Gene

Overview

Journal Genetics

Publisher Oxford University Press

Specialty Genetics

Date 2005 Mar 4

PMID 15744054

Citations 8

Authors

Kyle A Edgar

Marcia Belvin

Annette L Parks

Kellie Whittaker

Matt B Mahoney

Monique Nicoll

Christopher C Park

Christopher G Winter

Feng Chen

Kim Lickteig

Ferhad Ahmad

Hanife Esengil

Matthew V Lorenzi

Amanda Norton

Brent A Rupnow

Laleh Shayesteh

Mariano Tabios

Lynn M Young

Pamela M Carroll

Casey Kopczynski

Gregory D Plowman

Lori S Friedman

Helen L Francis-Lang

Affiliations

Soon will be listed here.

Abstract

Mutations that inactivate the retinoblastoma (Rb) pathway are common in human tumors. Such mutations promote tumor growth by deregulating the G1 cell cycle checkpoint. However, uncontrolled cell cycle progression can also produce new liabilities for cell survival. To uncover such liabilities in Rb mutant cells, we performed a clonal screen in the Drosophila eye to identify second-site mutations that eliminate Rbf(-) cells, but allow Rbf(+) cells to survive. Here we report the identification of a mutation in a novel highly conserved peptidyl prolyl isomerase (PPIase) that selectively eliminates Rbf(-) cells from the Drosophila eye.

Citing Articles

Cross-species identification of PIP5K1-, splicing- and ubiquitin-related pathways as potential targets for RB1-deficient cells.

Parkhitko A, Singh A, Hsieh S, Hu Y, Binari R, Lord C PLoS Genet. 2021; 17(2):e1009354.

PMID: 33591981 PMC: 7909629. DOI: 10.1371/journal.pgen.1009354.

Genome-wide synthetic lethal CRISPR screen identifies FIS1 as a genetic interactor of ALS-linked C9ORF72.

Chai N, Haney M, Couthouis J, Morgens D, Benjamin A, Wu K Brain Res. 2019; 1728:146601.

PMID: 31843624 PMC: 7539795. DOI: 10.1016/j.brainres.2019.146601.

Pleiotropic role of Drosophila phosphoribosyl pyrophosphate synthetase in autophagy and lysosome homeostasis.

Delos Santos K, Kim M, Yergeau C, Jean S, Moon N PLoS Genet. 2019; 15(9):e1008376.

PMID: 31487280 PMC: 6748441. DOI: 10.1371/journal.pgen.1008376.

Drosophila as a Potential Model for Ocular Tumors.

Bennett D, Lyulcheva E, Cobbe N Ocul Oncol Pathol. 2016; 1(3):190-9.

PMID: 27172095 PMC: 4847669. DOI: 10.1159/000370155.

Studying synthetic lethal interactions in the zebrafish system: insight into disease genes and mechanisms.

Hajeri V, Amatruda J Dis Model Mech. 2011; 5(1):33-7.

PMID: 22107871 PMC: 3255541. DOI: 10.1242/dmm.007989.

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