Kinetic Characterization of the Glycosyltransferase Module of Staphylococcus Aureus PBP2

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 2005 Mar 4

PMID 15743972

Citations 22

Authors

Dianah Barrett

Catherine Leimkuhler

Lan Chen

Deborah Walker

Daniel Kahne

Suzanne Walker

Affiliations

Soon will be listed here.

Abstract

We report the heterologous overexpression and purification of Staphylococcus aureus PBP2 and demonstrate efficient glycan polymerization from lipid II in vitro. S. aureus PBP2 is the first purified gram-positive class A penicillin-binding protein to show good transglycosylase activity. This enables further studies on this important class of enzymes.

Citing Articles

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Activities and regulation of peptidoglycan synthases.

Egan A, Biboy J, Vant Veer I, Breukink E, Vollmer W Philos Trans R Soc Lond B Biol Sci. 2015; 370(1679).

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Lipoprotein activators stimulate Escherichia coli penicillin-binding proteins by different mechanisms.

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