The Rok Protein of Bacillus Subtilis Represses Genes for Cell Surface and Extracellular Functions

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 2005 Mar 4

PMID 15743949

Citations 47

Authors

Mark Albano

Wiep Klaas Smits

Linh T Y Ho

Barbara Kraigher

Ines Mandic-Mulec

Oscar P Kuipers

David Dubnau

Affiliations

Soon will be listed here.

Abstract

Rok is a repressor of the transcriptional activator ComK and is therefore an important regulator of competence in Bacillus subtilis (T. T. Hoa, P. Tortosa, M. Albano, and D. Dubnau, Mol. Microbiol. 43:15-26, 2002). To address the wider role of Rok in the physiology of B. subtilis, we have used a combination of transcriptional profiling, gel shift experiments, and the analysis of lacZ fusions. We demonstrate that Rok is a repressor of a family of genes that specify membrane-localized and secreted proteins, including a number of genes that encode products with antibiotic activity. We present evidence for the recent introduction of rok into the B. subtilis-Bacillus licheniformis-Bacilllus amyloliquefaciens group by horizontal transmission.

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References

Long A, Mangalam H, Chan B, Tolleri L, Hatfield G, Baldi P . Improved statistical inference from DNA microarray data using analysis of variance and a Bayesian statistical framework. Analysis of global gene expression in Escherichia coli K12. J Biol Chem. 2001; 276(23):19937-44. DOI: 10.1074/jbc.M010192200. View

Liu X, Brutlag D, Liu J . BioProspector: discovering conserved DNA motifs in upstream regulatory regions of co-expressed genes. Pac Symp Biocomput. 2001; :127-38. View

Hoa T, Tortosa P, Albano M, Dubnau D . Rok (YkuW) regulates genetic competence in Bacillus subtilis by directly repressing comK. Mol Microbiol. 2002; 43(1):15-26. DOI: 10.1046/j.1365-2958.2002.02727.x. View

Berka R, Hahn J, Albano M, Draskovic I, Persuh M, Cui X . Microarray analysis of the Bacillus subtilis K-state: genome-wide expression changes dependent on ComK. Mol Microbiol. 2002; 43(5):1331-45. DOI: 10.1046/j.1365-2958.2002.02833.x. View

Ogura M, Yamaguchi H, Kobayashi K, Ogasawara N, Fujita Y, Tanaka T . Whole-genome analysis of genes regulated by the Bacillus subtilis competence transcription factor ComK. J Bacteriol. 2002; 184(9):2344-51. PMC: 134994. DOI: 10.1128/JB.184.9.2344-2351.2002. View

Dorenbos R, Stein T, Kabel J, Bruand C, Bolhuis A, Bron S . Thiol-disulfide oxidoreductases are essential for the production of the lantibiotic sublancin 168. J Biol Chem. 2002; 277(19):16682-8. DOI: 10.1074/jbc.M201158200. View

Hamoen L, Smits W, de Jong A, Holsappel S, Kuipers O . Improving the predictive value of the competence transcription factor (ComK) binding site in Bacillus subtilis using a genomic approach. Nucleic Acids Res. 2002; 30(24):5517-28. PMC: 140081. DOI: 10.1093/nar/gkf698. View

Goh E, Yim G, Tsui W, McClure J, Surette M, Davies J . Transcriptional modulation of bacterial gene expression by subinhibitory concentrations of antibiotics. Proc Natl Acad Sci U S A. 2002; 99(26):17025-30. PMC: 139263. DOI: 10.1073/pnas.252607699. View

Hamoen L, Kausche D, Marahiel M, Van Sinderen D, Venema G, Serror P . The Bacillus subtilis transition state regulator AbrB binds to the -35 promoter region of comK. FEMS Microbiol Lett. 2003; 218(2):299-304. DOI: 10.1111/j.1574-6968.2003.tb11532.x. View

10.

Kobayashi K, Ehrlich S, Albertini A, Amati G, Andersen K, Arnaud M . Essential Bacillus subtilis genes. Proc Natl Acad Sci U S A. 2003; 100(8):4678-83. PMC: 153615. DOI: 10.1073/pnas.0730515100. View

11.

Gonzalez-Pastor J, Hobbs E, Losick R . Cannibalism by sporulating bacteria. Science. 2003; 301(5632):510-3. DOI: 10.1126/science.1086462. View

12.

Bailey T, Noble W . Searching for statistically significant regulatory modules. Bioinformatics. 2003; 19 Suppl 2:ii16-25. DOI: 10.1093/bioinformatics/btg1054. View

13.

Susanna K, van der Werff A, den Hengst C, Calles B, Salas M, Venema G . Mechanism of transcription activation at the comG promoter by the competence transcription factor ComK of Bacillus subtilis. J Bacteriol. 2004; 186(4):1120-8. PMC: 344208. DOI: 10.1128/JB.186.4.1120-1128.2004. View

14.

Hamon M, Stanley N, Britton R, Grossman A, Lazazzera B . Identification of AbrB-regulated genes involved in biofilm formation by Bacillus subtilis. Mol Microbiol. 2004; 52(3):847-60. PMC: 1409746. DOI: 10.1111/j.1365-2958.2004.04023.x. View

15.

Tjalsma H, Antelmann H, Jongbloed J, Braun P, Darmon E, Dorenbos R . Proteomics of protein secretion by Bacillus subtilis: separating the "secrets" of the secretome. Microbiol Mol Biol Rev. 2004; 68(2):207-33. PMC: 419921. DOI: 10.1128/MMBR.68.2.207-233.2004. View

16.

Liu Y, Wei L, Batzoglou S, Brutlag D, Liu J, Liu X . A suite of web-based programs to search for transcriptional regulatory motifs. Nucleic Acids Res. 2004; 32(Web Server issue):W204-7. PMC: 441599. DOI: 10.1093/nar/gkh461. View

17.

Kodani S, Hudson M, Durrant M, Buttner M, Nodwell J, Willey J . The SapB morphogen is a lantibiotic-like peptide derived from the product of the developmental gene ramS in Streptomyces coelicolor. Proc Natl Acad Sci U S A. 2004; 101(31):11448-53. PMC: 509221. DOI: 10.1073/pnas.0404220101. View

18.

Albano M, Hahn J, Dubnau D . Expression of competence genes in Bacillus subtilis. J Bacteriol. 1987; 169(7):3110-7. PMC: 212357. DOI: 10.1128/jb.169.7.3110-3117.1987. View

19.

Magnuson R, Solomon J, Grossman A . Biochemical and genetic characterization of a competence pheromone from B. subtilis. Cell. 1994; 77(2):207-16. DOI: 10.1016/0092-8674(94)90313-1. View

20.

van Sinderen D, ten Berge A, Hayema B, Hamoen L, Venema G . Molecular cloning and sequence of comK, a gene required for genetic competence in Bacillus subtilis. Mol Microbiol. 1994; 11(4):695-703. DOI: 10.1111/j.1365-2958.1994.tb00347.x. View