Performance of Genomic Bordering Elements at Predefined Genomic Loci

Overview

Journal Mol Cell Biol

Specialty Cell Biology

Date 2005 Mar 4

PMID 15743822

Citations 22

Authors

Sandra Goetze

Alexandra Baer

Silke Winkelmann

Kristina Nehlsen

Jost Seibler

Karin Maass

Jurgen Bode

Affiliations

Soon will be listed here.

Abstract

Eukaryotic DNA is organized into chromatin domains that regulate gene expression and chromosome behavior. Insulators and/or scaffold-matrix attachment regions (S/MARs) mark the boundaries of these chromatin domains where they delimit enhancing and silencing effects from the outside. By recombinase-mediated cassette exchange (RMCE), we were able to compare these two types of bordering elements at a number of predefined genomic loci. Flanking an expression vector with either S/MARs or two copies of the non-S/MAR chicken hypersensitive site 4 insulator demonstrates that while these borders confer related expression characteristics at most loci, their effect on chromatin organization is clearly distinct. Our results suggest that the activity of bordering elements is most pronounced for the abundant class of loci with a low but negligible expression potential in the case of highly expressed sites. By the RMCE procedure, we demonstrate that expression parameters are not due to a potential targeting action of bordering elements, in the sense that a linked transgene is directed into a special class of loci. Instead, we can relate the observed transcriptional augmentation phenomena to their function as genomic insulators.

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